publication . Article . Other literature type . 2018

Time Course of Detection of Human Male DNA from Stained Blood Sample on Various Surfaces by Loop Mediated Isothermal Amplification and Polymerase Chain Reaction

Kanchanaphum, Panan;
Open Access English
  • Published: 01 Mar 2018 Journal: BioMed Research International (issn: 2314-6133, Copyright policy)
  • Publisher: Hindawi
Abstract
<jats:p>This study explores determining the sex of humans from blood stains taken from different surfaces and compares the time course of detection with the conventional PCR, Conventional Loop Mediated Isothermal Amplification (LAMP), and LAMP-Lateral Flow Dipstick (LFD). For the DNA templates, 7 male and 7 female blood stained samples were extracted and added to LAMP and PCR reaction solution to amplify the SRY gene. The DNA samples were extracted from the following blood stained materials: cloth, wood, clay, and tile. Then, the samples were stored at room temperature for 1, 7, 30, and 60 day(s). After the DNA amplification, the gel electrophoresis process was ...
Subjects
Medical Subject Headings: sense organseye diseasesgenetic structures
free text keywords: Article Subject, Research Article, Medicine, R, General Biochemistry, Genetics and Molecular Biology, General Immunology and Microbiology, General Medicine, Biochemistry, Gel electrophoresis, DNA, chemistry.chemical_compound, chemistry, Biology, Blood Stains, Chromatography, Dipstick, Loop-mediated isothermal amplification, Nucleic acid amplification technique, Polymerase chain reaction, law.invention, law, Blood stained
Related Organizations
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publication . Article . Other literature type . 2018

Time Course of Detection of Human Male DNA from Stained Blood Sample on Various Surfaces by Loop Mediated Isothermal Amplification and Polymerase Chain Reaction

Kanchanaphum, Panan;