publication . Article . 2017

Regulation of Calvarial Osteogenesis by Concomitant De-repression of GLI3 and Activation of IHH Targets.

Lotta K. Veistinen; Tuija Mustonen; Tuija Mustonen; Md. Rakibul Hasan; Maarit Takatalo; Yukiho Kobayashi; Yukiho Kobayashi; Dörthe A. Kesper; Andrea Vortkamp; David P. Rice; ...
Open Access English
  • Published: 01 Dec 2017 Journal: Frontiers in Physiology (issn: 1664-042X, Copyright policy)
  • Publisher: Frontiers Media S.A.
  • Country: Finland
Abstract
Loss-of-function mutations in GLI3 and IHH cause craniosynostois and reduced osteogeneiss, respectively. In this study, we show that ihh ligand, the receptor Ptch1 and Gli transcription factors are differentialyy expressed in embryonic mouse calvaria osteogenic condenstions. We show that in both ihh(-/-) and Gli3(Xt-J/Xt-J) embroyonic mice, the normal gene expression architecture is lost and this results in disorganized calvarial bone developement. RUNX2 is a master regulatory transciption factor controlling osteogenesis. In the absence of Gli3, RUNX2 isoform II and IHH are upregulated, and RUNX2 isoform I downregulated. This is consistent with the expandeed and...
Subjects
Medical Subject Headings: body regionsmusculoskeletal diseasesembryonic structuresstomatognathic system
free text keywords: calvarial development, hedgehog signaling pathway, osteoblast, cell differentiation, craniosynostosis, Physiology, QP1-981, Physiology (medical), Original Research, BONE-DEVELOPMENT, INDIAN-HEDGEHOG, OSTEOBLAST DIFFERENTIATION, EXPRESSION PATTERNS, DIGIT NUMBER, DERMAL BONE, STEM-CELLS, MOUSE, 313 Dentistry, 1184 Genetics, developmental biology, physiology, Cell biology, Indian hedgehog, biology.organism_classification, biology, Cranial suture morphogenesis, Calvaria, medicine.anatomical_structure, medicine, Cellular differentiation, RUNX2, GLI3, Transcription factor
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