publication . Article . 2014

Impedimetric Urea Biosensor Based on Modified Gold Electrode with Urease Immobilized on Glutathione Layer

Houcine BARHOUMI; Abderrazak MAAREF; Nicole JAFFREZIC-RENAULT;
Open Access English
  • Published: 01 May 2014 Journal: Sensors & Transducers (issn: 2306-8515, eissn: 1726-5479, Copyright policy)
  • Publisher: IFSA Publishing, S.L.
Abstract
In this work, a glutathione (GSH) modified gold microelectrode was used for the covalent immobilization of urease biomolecules via the glutaraldehyde-coupling agent. The self- assembled monolayers (SAMs) onto the gold surface was investigated by using the electrochemical impedance spectroscopy measurements (EIS). Before urease grafting, a significant interaction was noticed between urea and the glutathione layer by forming hydrogen bonds. The H-NMR analysis was carried out to highlight the possibility of having a covalent link between urea and the GSH deposited layer. In addition, contact angle measurements were carried out to determine the hydrophobic/hydrophil...
Subjects
free text keywords: Glutathione SAMs, Urea-GSH interaction, Urease immobilization, Impedance spectroscopy, Urea biosensor., Technology (General), T1-995
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35 references, page 1 of 3

[1]. T. Kong, Y. Chen, Y. Ye, K. Zhang, Z. Wang, X. Wang, An amperometric glucose biosensor based on the immobilization of glucose oxidase on the ZnO nanotubes, Sensors and Actuators B, Vol. 138, 2009, pp. 344-350.

[2]. S. Brahim, D. Narinesingh, A. Guiseppi-Elie, Amperometric determination of cholesterol in serum using a cholesterol oxidase biosensor with a polypyrrole / hydrogel membrane, Analytica Chimica Acta, Vol. 448, 2001, pp. 27-36. [OpenAIRE]

[3]. M. Stredánský, A. Pizzariello, S. Stredánská, S. Miertuš, pH-sensing biosensors for urea, penicillin and oxalacetate, Analytica Chimica Acta, Vol. 415, 2000, pp. 151-157.

[4]. T. Shimomura, T. Itoh, T. Sumiya, F. Mizukami, M. Ono, Amperometric biosensor based on enzymes immobilized in hybrid mesoporous membranes for the determination of acetylcholine, Enzyme and Microbial Technology, Vol. 45, 2009, pp. 443-448.

[5]. S. Yadav, A. Kumar, C. S. Pundir, Amperometric creatinine biosensor based on covalently coimmobilized enzymes onto carboxylated multiwalled carbon nanotubes/polyaniline composite film, Analytical Biochemistry, Vol. 419, 2011, pp. 277-283.

[6]. H. Barhoumi, A. Maaref, M. Rammah, C. Martelet, N. Jaffrezic-Renault, C. Mousty, S. Cosnier, E. Perezet I. Rico-Lattes, Insulator semiconductor structures coated with biodegradable latexes as encapsulation matrix for urease, Biosensors and Bioelectronics, Vol. 20, 2005, pp. 2318-2323. [OpenAIRE]

[7]. D. Brondani, C. W. Scheeren, J. Dupont, I. Cruz Vieira, Biosensor based on platinum nanoparticles dispersed in ionic liquid and laccase for determination of adrenaline, Sensors and Actuators B, Vol. 140, 2009, pp. 252-259. [OpenAIRE]

[8]. L. I. B. Silvaa, F. D. P. Ferreira, A. C. Freitas, T. A. P. Rocha-Santos, A.C. Duarte, Optical fiber biosensor coupled to chromatographic separation for screening of dopamine, norepinephrine and epinephrine in human urine and plasma, Talanta, Vol. 80, 2009, pp. 853-857.

[9]. D. M. Sullivan, J. L. Havlin, Flow injection analysis of urea nitrogen in soil extracts, Soil Sci. Soc. Am. J, Vol. 55, 1991, pp. 109-113.

[10]. J. Gonzalez-Rodriguez, P. Perez-Juan, M. D.L. de Castro, Method for monitoring urea and ammonia in wine and must by flow injection-pervaporation, Anal. Chim. Acta., Vol. 471, 2002, pp. 105-111.

[11]. J. L. F. C. Lima, C. Delerue-Matos, M. C. V. F. Vaz, J. Agric, Flow injection system with potentiometric detection for the determination of urea content in milks, Food Chem. Vol. 46, 1998, pp. 1386-1389. [OpenAIRE]

[12]. X. C. Hu, M. Takenaka, M. Kitano, H. Bandow, Y. Maeda, M. Hattori, Determination of trace amounts of urea by using flow injection with chemiluminescence detection, Analyst, Vol. 119, 1994, pp. 1829-1833. [OpenAIRE]

[13]. D. Chirizzi, C. Malitesta, Potentiometric urea biosensor based on urease immobilized by an electrosynthesized poly(o-phenylenediamine) film with buffering capabi, Sensors and Actuators B , Vol. 157, 2011, pp. 211-215. [OpenAIRE]

[14]. Y. Velichkovaa, Y. Ivanova, I. Marinova, R. Rameshb, N. R. Kaminib, N. Dimchevac, E. Horozovac, T. Godjevargovaa, Amperometric electrode for determination of urea using electrodeposited rhodium and immobilized urease, Journal of Molecular Catalysis B, Vol. 69, 2011, pp. 168-175.

[15]. W. O. Ho, S. Krause, C. J. McNeil, J. A. Pritchard, R. D. Armstrong, D. Athey, K. Rawson, Electrochemical sensor for measurement of urea and creatinine in serum based on ac impedance measurement of enzyme-catalyzed polymer transformation, Anal. Chem., Vol. 71, 1999, pp. 1940-1946.

35 references, page 1 of 3
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