publication . Article . 2014

Impedimetric Urea Biosensor Based on Modified Gold Electrode with Urease Immobilized on Glutathione Layer

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  • Published: 01 May 2014 Journal: Sensors & Transducers (issn: 2306-8515, eissn: 1726-5479, Copyright policy)
  • Publisher: IFSA Publishing, S.L.
In this work, a glutathione (GSH) modified gold microelectrode was used for the covalent immobilization of urease biomolecules via the glutaraldehyde-coupling agent. The self- assembled monolayers (SAMs) onto the gold surface was investigated by using the electrochemical impedance spectroscopy measurements (EIS). Before urease grafting, a significant interaction was noticed between urea and the glutathione layer by forming hydrogen bonds. The H-NMR analysis was carried out to highlight the possibility of having a covalent link between urea and the GSH deposited layer. In addition, contact angle measurements were carried out to determine the hydrophobic/hydrophil...
free text keywords: Glutathione SAMs, Urea-GSH interaction, Urease immobilization, Impedance spectroscopy, Urea biosensor., Technology (General), T1-995
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