publication . Article . 2009

Detection of Cryptosporidium sp infection by PCR and modified acid fast staining from potassium dichromate preserved stool

Herbowo A. Soetomenggolo; Sri W. Dwintasari; Septelia Inawati Wanandi; Agnes Kurniawan;
Open Access
  • Published: 01 Aug 2009 Journal: Medical Journal of Indonesia (issn: 0853-1773, eissn: 2252-8083, Copyright policy)
  • Publisher: Faculty of Medicine, Universitas Indonesia
<p><strong>Aim</strong> To identify the frequency of <em>Cryptosporidium</em> infection in children below 3 years old by examining concentrated long term preserved stool using PCR detection of 18S rRNA gene and compared with modified acid fast staining technique.</p><p><strong>Methods</strong> Hundred eighty eight stools from children ≤ 3 years old were stored for 13 months in 2.5% K2Cr2O7 solution at 40C. <em>Cryptosporidium</em> oocysts were isolated by water-ether concentration technique. The concentrates were smeared onto object glass and stained with modified acid fast staining, and the rest of the concentrates were DNA extracted by freezing and thawing cyc...
free text keywords: General Medicine, Medicine (General), R5-920, Cryptosporidium, biology.organism_classification, biology, Molecular biology, Cryptosporidium sp, Acid-fast, Proteinase K, biology.protein, Digestion, Potassium dichromate, chemistry.chemical_compound, chemistry, Microbiology
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