publication . Article . 2011

A Western-blot assay for the detection of antibodies against pathogenic Leptospira serogroups with recombinant outer membrane protein LipL32

Hong-yuan DUAN; Zhi-guo LIU; Shao-fu QIU; Bin HE; Hai ZHAO; Li-hua SONG; Hong ZHU; Qing DUAN;
Open Access Chinese
  • Published: 01 Mar 2011 Journal: Medical Journal of Chinese People's Liberation Army (issn: 0577-7402, Copyright policy)
  • Publisher: Editorial Board of Medical Journal of Chinese People's Liberation Army
Abstract
Objective To provide a possible antigen for rapid serodiagnosis of leptospirosis,the present study focused on the activity of immune-reaction and cross-reaction between outer membrane protein LipL32 and multi-serogroup anti-pathogenic Leptospira antibodies.Methods Based on the given sequence of LipL32 gene of Leptospira icterohaemorrhagiae strain 56601,the primer pair was designed and the DNA fragment was amplified by PCR.The amplified product was inserted into vector pET-28a-(c) to construct a recombinant plasmid.With E.coli BL21(DE3),the recombinant protein was induced for expression.After purification,the Western-blot assay was applied as a method to evaluate...
Subjects
Medical Subject Headings: bacteriabacterial infections and mycosesanimal diseases
free text keywords: Leptospira interrogans serovar icterohaemorrhagiae; antibody; cross reactions, Medicine, R, Medicine (General), R5-920
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