publication . Article . 2007

Comparison of Methods for Isolating High Quality DNA and RNA from an Oleaginous Fungus Cunninghamella bainieri Strain 2a1

Noor Adila, A. K.; Farah Diba, A. B.; Zamri, Z.; Wan Mohtar, W. Y.; Aidil, A. H.; Mahadi, N. M.; Murad, A. M. A.;
Open Access
  • Published: 01 Jan 2007 Journal: Malaysian Journal of Microbiology (issn: 2231-7538, Copyright policy)
  • Publisher: Malaysian Journal of Microbiology
Abstract
A number of protocols have been reported for efficient fungal DNA and RNA isolation. However, many of these methods are often designed for certain groups or morphological forms of fungi and, in some cases, are species dependent. In this report, we compared four published protocols for DNA isolation from a locally isolated oleaginous fungus, Cunninghamella bainieri strain 2a1. These protocols either involved the use of polyvinyl pyrrolidone (PVP), hexacetyltrimethylammonium bromide (CTAB) or without using PVB or CTAB. For RNA isolation, we tested two published protocols, one of which is based on TRI REAGENT (Molecular Research Center, USA) and another is simple m...
Subjects
free text keywords: Microbiology (medical), Infectious Diseases, Cunninghamella bainieri, DNA extraction, RNA extraction, Microbiology, QR1-502
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