publication . Article . 2018

Development of ent-kaurene oxidase-based conserved intron spanning primers for species identification in the genus poa (poaceae; bluegrass)

Jonathan LaMantia; Ambika Chandra; David Huff;
Open Access English
  • Published: 01 Apr 2018 Journal: Agronomy (issn: 2073-4395, Copyright policy)
  • Publisher: MDPI AG
Abstract
Interspecific hybridization has been attempted to combine the heat and drought of Poa arachnifera Torr. with the turf quality characteristics of several Poa species. Confirmation of an F1 hybrid through morphological analysis of vegetative and flowering characteristics is often time consuming and ambiguous. Ent-kaurene oxidase (KO) has been sequenced in rice, barley, and wheat. In rice, each of the five copies of KO gene has unique lengths for the first intron. Conserved intron spanning primers (CISP) can be used as a DNA marker to exploit variations of intron lengths that flank conserved gene sequences. In the present study, we developed CISP to sequence partia...
Subjects
Medical Subject Headings: food and beveragesendocrine system
free text keywords: Texas bluegrass, turfgrass, molecular marker, Agriculture, S, chemistry.chemical_compound, chemistry, Poa arachnifera, biology.organism_classification, biology, Hybrid, Poaceae, Gene, Botany, Sequence analysis, Primer (molecular biology), Intron
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Article . 2018
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Article . 2018
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publication . Article . 2018

Development of ent-kaurene oxidase-based conserved intron spanning primers for species identification in the genus poa (poaceae; bluegrass)

Jonathan LaMantia; Ambika Chandra; David Huff;