publication . Article . 2006

Inmunofluorescencia con Crithidia luciliae para la detección de anticuerpos anti-ADN: Imágenes atípicas y su relación con enfermedad de Chagas y leishmaniasis Immunofluorescence assay with Crithidia luciliae for the detection of anti-DNA antibodies: Atypical images and their relationship with Chagas’ disease and leishmaniasis

Gloria Griemberg; Nidia F. Ferrarotti; Graciela Svibel; Maria R. Ravelli; Néstor J. Taranto; Emilio L. Malchiodi; María C. Pizzimenti;
Open Access English
  • Published: 01 Feb 2006 Journal: Medicina (Buenos Aires) (issn: 0025-7680, eissn: 1669-9106, Copyright policy)
  • Publisher: Fundación Revista Medicina
Abstract
Los anticuerpos anti-ADN nativo pueden detectarse por inmunofluorescencia indirecta con Crithidia luciliae, observándose tinción fluorescente anular del kinetoplasto que contiene ADN de doble cadena. En algunos casos pueden observarse imágenes fluorescentes en flagelo, membrana y corpúsculo basal, consideradas atípicas. Como C. luciliae pertenece a la familia Trypanosomatidae, que incluye patógenos para el hombre como Trypanosoma cruzi y Leishmaniaspp., se consideró que las imágenes atípicas pudieran deberse a reacciones cruzadas. Se realizaron estudios serológicos para Chagas a 105 muestras provenientes de zona endémica (Corrientes) y no endémica (Buenos Aires)...
Subjects
Medical Subject Headings: parasitic diseases
free text keywords: enfermedad de Chagas, leishmaniasis, Crithidia luciliae, anticuerpos anti-ADN, Chagas’ disease, anti-DNA antibodies, Medicine, R, Immunologic diseases. Allergy, RC581-607, Infectious and parasitic diseases, RC109-216
Download from

3. Tan N, Cohen AS, Fries JF, et al. Special article. The 1982 revised criteria for the classification of systemic lupus erythematosus. Arthritis Rheum 1982; 25: 1271-7.

4. Hochberg MC. Updating the American College of Rheumatology revised criteria for the classification of systemic lupus erythemotosus. Arthritis Rheum 1997; 9: 1725.

5. Tan EM. Antinuclear antibodies: diagnostic markers for autoimmune diseases and probes for cell biology. Adv Immunol 1989; 44: 93-151.

6. Swaak AJ, Aarden LA, Statius van Eps LW, Feltkamp TE. Anti-dsDNA and complement profiles as prognostic guides in systemic lupus erythematosus. Arthritis Rheum 1979; 22: 226-35.

7. Aarden LA, Lakmaker F, de Groot ER, Swaak AJ, Feltkamp TE. Detection of antibodies to DNA by radioimmunoassay and immunofluorescence. Scand J Rheumatol. Suppl 1975; 11: 12-9.

8. Rubin RL, Jolin FG, Tan EM. An improved ELISA for antinature DNA by elimination of interference by anti-histone antibodies. J Immunol Methods 1983; 63: 359-66.

9. Ballou SP. Crithidia luciliae immunofluorescent test for antibodies to DNA. In: Rose NR, Conway de Macario E, Fahey JL, Friedman H, Penn GM. (eds). Manual of Clinical Laboratory Immunology, 4th ed. Washington D.C: American Society for Microbiology, 1992, p 730-4.

10. Malchiodi EL, Chiaramonte MG, Taranto NJ, Zwirner NW, Margni RA. Cross-reactivity studies and diferencial serodiagnosis of human infections caused by Trypanosoma cruzi and Leishmania spp: the use of immunoblotting and ELISA with a purified antigen (Ag 163B6). Clin Exp Immunol 1994; 97: 417-23.

11. Lopes JD, Caulada Z, Barbari CL, Plessmann Camargo E. Cross-reactivity between Trypanosoma cruzi and insect trypanosomatids as a basis for the diagnosis of Chagas' disease. Am J Trop Med Hyg 1981; 30: 1183-8. [OpenAIRE]

12. Chiaramonte MG, Zwirner NW, Caropresi SL, Taranto NJ, Malchiodi EL. Trypanosoma cruzi and Leishmania spp. human mixed infection. Am J Trop Med Hyg 1996; 54: 271-3. [OpenAIRE]

13. Chiaramonte MG, Frank FM, Furer GM, Taranto NJ, Margni RA, Malchiodi EL. Polymerase chain reaction reveals Trypanosoma cruzi infection suspected by serology in leishmaniasis patients. Acta Tropica 1999; 72: 295-308.

14. Frank FM, Fernández MM, Taranto NJ, et al. Characterization of human infection by Leishmania spp. in the Northwest of Argentina: immune response, double infection with Trypanosoma cruzi and species of Leishmania involved. Parasitology 2003; 126: 31-9.

15. Monteón VM, Guzmán-Rojas L, Negrete-García C, Rosales-Encina JL, Reyes López PA. Serodiagnosis of American Trypanosomosis by using non pathogenic trypanosomatid antigen. J Clin Microbiol 1997; 35: 3316-9. [OpenAIRE]

16. Chiaramonte MG, Zwirner NW, Caropresi SL, Heredia V, Taranto NJ, Malchiodi EL. Human leishmaniasis infection in the province of Salta. Evidence of mixed infection with Trypanosoma cruzi and Leishmania spp. Medicina (Buenos Aires) 1996; 56: 259-68. [OpenAIRE]

Powered by OpenAIRE Research Graph
Any information missing or wrong?Report an Issue