publication . Article . 2015

Characterization of the serine acetyltransferase gene family of Vitis vinifera uncovers differences in regulation of OAS synthesis in woody plants.

Silvia eTavares; Silvia eTavares; Markus eWirtz; Marcel Pascal Beier; Jochen eBogs; Jochen eBogs; Jochen eBogs; Ruediger eHell; Sara eAmâncio;
Open Access English
  • Published: 17 Feb 2015 Journal: Frontiers in Plant Science (issn: 1664-462X, Copyright policy)
  • Publisher: Frontiers Media S.A.
  • Country: Portugal
Abstract
In higher plants cysteine biosynthesis is catalyzed by O-acetylserine(thiol)lyase (OASTL) and represents the last step of the assimilatory sulfate reduction pathway. It is mainly regulated by provision of O-acetylserine (OAS), the nitrogen/carbon containing backbone for fixation of reduced sulfur. OAS is synthesized by Serine acetyltransferase (SERAT), which reversibly interacts with OASTL in the cysteine synthase complex (CSC). In this study we identify and characterize the SERAT protein family of the crop plant Vitis vinifera. The identified four members of the VvSERAT gene family are assigned to three distinct groups upon their sequence similarities to Arabid...
Subjects
free text keywords: drought, Vitis vinifera, S deficiency, SERAT, OAS (O-acetylserine), Plant culture, SB1-1110, OAS, Plant Science, Original Research Article, Lyase, Protein family, Biology, Plastid, Biochemistry, Gene family, Arabidopsis, biology.organism_classification, Cysteine, Cytosol, Cysteine synthase complex
Funded by
FCT| SFRH/BPD/65965/2009
Project
SFRH/BPD/65965/2009
IDENTIFICATION AND CHARACTERISATION OF SMALL SECRETED PROTEINS OF THE COFFEE LEAF RUST PATHOGEN (HEMILEIA VASTATRIX)
  • Funder: Fundação para a Ciência e a Tecnologia, I.P. (FCT)
  • Project Code: SFRH/BPD/65965/2009
  • Funding stream: SFRH | Pós-Doutoramento
84 references, page 1 of 6

An, Y. Q., McDowell, J. M., Huang, S., McKinney, E. C., Chambliss, S., and Meager, R. B. (1996). Strong, constitutive expression of the Arabidopsis ACT2/ACT8 actin subclass in vegetative tissues. Plant J. 10, 107-121. doi: 10.1046/j.1365- 313X.1996.10010107.x [OpenAIRE]

Berkowitz, O., Wirtz, M., Wolf, A., Kuhlmann, J., and Hell, R. (2002). Use of biomolecular interaction analysis to elucidate the regulatory mechanism of the cysteine synthase complex from Arabidopsis thaliana. J. Biol. Chem. 277, 30629-30634. doi: 10.1074/jbc.M111632200

Birke, H., Haas, F. H., De Kok, L. J., Balk, J., Wirtz, M., and Hell, R. (2012a). Cysteine biosynthesis, in concert with a novel mechanism, contributes to sulfide detoxification in mitochondria of Arabidopsis thaliana. Biochem. J. 445, 275-283. doi: 10.1042/BJ20120038

Birke, H., Heeg, C., Wirtz, M., and Hell, R. (2013). Successful fertilization requires the presence of at least one major O-Acetylserine(thiol)lyase for cysteine synthesis in pollen of Arabidopsis. Plant Physiol. 163, 959-972. doi: 10.1104/pp.113.221200 [OpenAIRE]

Birke, H., Muller, S. J., Rother, M., Zimmer, A. D., Hoernstein, S. N., Wesenberg, D., et al. (2012b). The relevance of compartmentation for cysteine synthesis in phototrophic organisms. Protoplasma 249(Suppl. 2), 147-155. doi: 10.1007/s00709-012-0411-9

Blaszczyk, A., Brodzik, R., and Sirko, A. (1999). Increased resistance to oxidative stress in transgenic tobacco plants overexpressing bacterial serine acetyltransferase. Plant J. 20, 237-243. doi: 10.1046/j.1365-313x.1999.00596.x

Bradford, M. M. (1976). A rapid and sensitive method for the quantitation of microgram quantities of protein utilizing the principle of protein-dye binding. Anal. Biochem. 72, 248-254. doi: 10.1016/0003-2697(76)90527-3

Brunold, C., and Suter, M. (1982). Intracellular localization of serine acetyltransferase in spinach leaves. Planta 155, 321-327. doi: 10.1007/BF004 29459 [OpenAIRE]

Cao, M. J., Wang, Z., Zhao, Q., Mao, J. L., Speiser, A., Wirtz, M., et al. (2014). Sulfate availability affects ABA levels and germination response to ABA and salt stress in Arabidopsis thaliana. Plant J. 77, 604-615. doi: 10.1111/tpj.12407

Chan, K. X., Wirtz, M., Phua, S. Y., Estavillo, G. M., and Pogson, B. J. (2013). Balancing metabolites in drought: the sulfur assimilation conundrum. Trends Plant Sci. 18, 18-29. doi: 10.1016/j.tplants.2012.07.005

Coito, J. L., Rocheta, M., Carvalho, L., and Amâncio, S. (2012). Microarray-based uncovering reference genes for quantitative real time PCR in grapevine under abiotic stress. BMC Res. Notes 5:220. doi: 10.1186/1756-0500-5-220

Davidian, J. C., and Kopriva, S. (2010). Regulation of sulfate uptake and assimilation-the same or not the same? Mol. Plant 3, 314-325. doi: 10.1093/mp/ssq001 [OpenAIRE]

Dominguez-Solis, J. R., He, Z., Lima, A., Ting, J., Buchanan, B. B., and Luan, S. (2008). A cyclophilin links redox and light signals to cysteine biosynthesis and stress responses in chloroplasts. Proc. Natl. Acad. Sci. U.S.A. 105, 16386-16391. doi: 10.1073/pnas.0808204105

Emanuelsson, O., Nielsen, H., Brunak, S., and Von Heijne, G. (2000). Predicting subcellular localization of proteins based on their N-terminal amino acid sequence. J. Mol. Biol. 300, 1005-1016. doi: 10.1006/jmbi.2000.3903 [OpenAIRE]

Emanuelsson, O., Nielsen, H., and Von Heijne, G. (1999). ChloroP, a neural network-based method for predicting chloroplast transit peptides and their cleavage sites. Protein Sci. 8, 978-984. doi: 10.1110/ps.8.5.978 [OpenAIRE]

84 references, page 1 of 6
Related research
Abstract
In higher plants cysteine biosynthesis is catalyzed by O-acetylserine(thiol)lyase (OASTL) and represents the last step of the assimilatory sulfate reduction pathway. It is mainly regulated by provision of O-acetylserine (OAS), the nitrogen/carbon containing backbone for fixation of reduced sulfur. OAS is synthesized by Serine acetyltransferase (SERAT), which reversibly interacts with OASTL in the cysteine synthase complex (CSC). In this study we identify and characterize the SERAT protein family of the crop plant Vitis vinifera. The identified four members of the VvSERAT gene family are assigned to three distinct groups upon their sequence similarities to Arabid...
Subjects
free text keywords: drought, Vitis vinifera, S deficiency, SERAT, OAS (O-acetylserine), Plant culture, SB1-1110, OAS, Plant Science, Original Research Article, Lyase, Protein family, Biology, Plastid, Biochemistry, Gene family, Arabidopsis, biology.organism_classification, Cysteine, Cytosol, Cysteine synthase complex
Funded by
FCT| SFRH/BPD/65965/2009
Project
SFRH/BPD/65965/2009
IDENTIFICATION AND CHARACTERISATION OF SMALL SECRETED PROTEINS OF THE COFFEE LEAF RUST PATHOGEN (HEMILEIA VASTATRIX)
  • Funder: Fundação para a Ciência e a Tecnologia, I.P. (FCT)
  • Project Code: SFRH/BPD/65965/2009
  • Funding stream: SFRH | Pós-Doutoramento
84 references, page 1 of 6

An, Y. Q., McDowell, J. M., Huang, S., McKinney, E. C., Chambliss, S., and Meager, R. B. (1996). Strong, constitutive expression of the Arabidopsis ACT2/ACT8 actin subclass in vegetative tissues. Plant J. 10, 107-121. doi: 10.1046/j.1365- 313X.1996.10010107.x [OpenAIRE]

Berkowitz, O., Wirtz, M., Wolf, A., Kuhlmann, J., and Hell, R. (2002). Use of biomolecular interaction analysis to elucidate the regulatory mechanism of the cysteine synthase complex from Arabidopsis thaliana. J. Biol. Chem. 277, 30629-30634. doi: 10.1074/jbc.M111632200

Birke, H., Haas, F. H., De Kok, L. J., Balk, J., Wirtz, M., and Hell, R. (2012a). Cysteine biosynthesis, in concert with a novel mechanism, contributes to sulfide detoxification in mitochondria of Arabidopsis thaliana. Biochem. J. 445, 275-283. doi: 10.1042/BJ20120038

Birke, H., Heeg, C., Wirtz, M., and Hell, R. (2013). Successful fertilization requires the presence of at least one major O-Acetylserine(thiol)lyase for cysteine synthesis in pollen of Arabidopsis. Plant Physiol. 163, 959-972. doi: 10.1104/pp.113.221200 [OpenAIRE]

Birke, H., Muller, S. J., Rother, M., Zimmer, A. D., Hoernstein, S. N., Wesenberg, D., et al. (2012b). The relevance of compartmentation for cysteine synthesis in phototrophic organisms. Protoplasma 249(Suppl. 2), 147-155. doi: 10.1007/s00709-012-0411-9

Blaszczyk, A., Brodzik, R., and Sirko, A. (1999). Increased resistance to oxidative stress in transgenic tobacco plants overexpressing bacterial serine acetyltransferase. Plant J. 20, 237-243. doi: 10.1046/j.1365-313x.1999.00596.x

Bradford, M. M. (1976). A rapid and sensitive method for the quantitation of microgram quantities of protein utilizing the principle of protein-dye binding. Anal. Biochem. 72, 248-254. doi: 10.1016/0003-2697(76)90527-3

Brunold, C., and Suter, M. (1982). Intracellular localization of serine acetyltransferase in spinach leaves. Planta 155, 321-327. doi: 10.1007/BF004 29459 [OpenAIRE]

Cao, M. J., Wang, Z., Zhao, Q., Mao, J. L., Speiser, A., Wirtz, M., et al. (2014). Sulfate availability affects ABA levels and germination response to ABA and salt stress in Arabidopsis thaliana. Plant J. 77, 604-615. doi: 10.1111/tpj.12407

Chan, K. X., Wirtz, M., Phua, S. Y., Estavillo, G. M., and Pogson, B. J. (2013). Balancing metabolites in drought: the sulfur assimilation conundrum. Trends Plant Sci. 18, 18-29. doi: 10.1016/j.tplants.2012.07.005

Coito, J. L., Rocheta, M., Carvalho, L., and Amâncio, S. (2012). Microarray-based uncovering reference genes for quantitative real time PCR in grapevine under abiotic stress. BMC Res. Notes 5:220. doi: 10.1186/1756-0500-5-220

Davidian, J. C., and Kopriva, S. (2010). Regulation of sulfate uptake and assimilation-the same or not the same? Mol. Plant 3, 314-325. doi: 10.1093/mp/ssq001 [OpenAIRE]

Dominguez-Solis, J. R., He, Z., Lima, A., Ting, J., Buchanan, B. B., and Luan, S. (2008). A cyclophilin links redox and light signals to cysteine biosynthesis and stress responses in chloroplasts. Proc. Natl. Acad. Sci. U.S.A. 105, 16386-16391. doi: 10.1073/pnas.0808204105

Emanuelsson, O., Nielsen, H., Brunak, S., and Von Heijne, G. (2000). Predicting subcellular localization of proteins based on their N-terminal amino acid sequence. J. Mol. Biol. 300, 1005-1016. doi: 10.1006/jmbi.2000.3903 [OpenAIRE]

Emanuelsson, O., Nielsen, H., and Von Heijne, G. (1999). ChloroP, a neural network-based method for predicting chloroplast transit peptides and their cleavage sites. Protein Sci. 8, 978-984. doi: 10.1110/ps.8.5.978 [OpenAIRE]

84 references, page 1 of 6
Related research
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