publication . Article . 2016

Isolation of new gravitropic mutants under hypergravity conditions

Akiko Mori; Masatsugu Toyota; Masatsugu Toyota; Masayoshi Shimada; Mika Mekata; Tetsuya Kurata; Masao Tasaka; Miyo Terao Morita;
Open Access English
  • Published: 01 Sep 2016 Journal: Frontiers in Plant Science (issn: 1664-462X, Copyright policy)
  • Publisher: Frontiers Media S.A.
Abstract
Forward genetics is a powerful approach used to link genotypes and phenotypes, and mutant screening/analysis has provided deep insights into many aspects of plant physiology. Gravitropism is a tropistic response in plants, in which hypocotyls and stems sense the direction of gravity and grow upwards. Previous studies of gravitropic mutants have suggested that shoot endodermal cells in Arabidopsis stems and hypocotyls are capable of sensing gravity (i.e., statocytes). In the present study, we report a new screening system using hypergravity conditions to isolate enhancers of gravitropism mutants, and we also describe a rapid and efficient genome mapping method, u...
Subjects
free text keywords: Gravitropism, Hypergravity, Arabidopsis thaliana, whole genome sequencing, Enhancer mutant, Plant culture, SB1-1110
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