publication . Article . 2018

Performance of Molecular Approaches for Aspergillus Detection and Azole Resistance Surveillance in Cystic Fibrosis

Hélène Guegan; Sylviane Chevrier; Chantal Belleguic; Eric Deneuville; Florence Robert-Gangneux; Florence Robert-Gangneux; Jean-Pierre Gangneux; Jean-Pierre Gangneux;
Open Access English
  • Published: 27 Mar 2018 Journal: Frontiers in Microbiology, volume 9 (eissn: 1664-302X, Copyright policy)
  • Publisher: Frontiers Media S.A.
  • Country: France
Abstract
International audience; Aspergillus fumigatus triazole resistance is an emerging concern for treating chronically infected/colonized patients. This study sought to evaluate the performance of PCR assays to detect Aspergillus fungi together with azole resistance in sputum samples from cystic fibrosis (CF) patients. In total, 119 sputum samples from 87 CF patients were prospectively processed for Aspergillus detection by means of mycological culture and four qPCR assays, 2 in-house methods and two commercial multiplex real-time PCR assays simultaneously detecting Aspergillus and the most relevant cyp51A gene mutations (MycoGENIE (R) and AsperGenius (R)). Azole sus...
Subjects
free text keywords: Microbiology, Original Research, Aspergillus, aspergillosis, cystic fibrosis, quantitative real-time PCR, azole resistance, sputum, [SDV.EE.SANT]Life Sciences [q-bio]/Ecology, environment/Health, QR1-502, Microbiology (medical), Biology, biology.organism_classification, medicine.disease, medicine, medicine.symptom, Multiplex, Aspergillus fumigatus, Gene mutation, Azole, chemistry.chemical_classification, chemistry
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publication . Article . 2018

Performance of Molecular Approaches for Aspergillus Detection and Azole Resistance Surveillance in Cystic Fibrosis

Hélène Guegan; Sylviane Chevrier; Chantal Belleguic; Eric Deneuville; Florence Robert-Gangneux; Florence Robert-Gangneux; Jean-Pierre Gangneux; Jean-Pierre Gangneux;