publication . Article . 2011

Effect of Vitrification on Sperm Parameters and Apoptosis in Fertile Men

M Adib; M Ramezani; MA Khalili;
Open Access Persian
  • Published: 01 Jan 2011 Journal: Armaghane Danesh Bimonthly Journal (issn: 1728-6506, eissn: 1728-6514, Copyright policy)
  • Publisher: Yasuj University Of Medical Sciences
Introduction & Objective: Today, cryopreservation of the human sperm is a common technique for treating infertility. It has been indicated that cryopreservation by different methods decrease the sperm motility and viability in fertile men, but still effect of freezing of the sperm by vitrification method have not been evaluated on sperm parameters and apoptosis. The aim of this study was to evaluate the effect of vitrification of sperm of fertile men on different sperm parameters (motility, morphology, viability and count) and apoptosis after thawing. Materials & Methods: In this experimental study which was conducted at Yazd Infertility Research and Clinical Ce...
Medical Subject Headings: urogenital systemendocrine systemreproductive and urinary physiology
free text keywords: Vitrification, Sperm, Apoptosis, fertility, Medicine (General), R5-920
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Article . 2011
25 references, page 1 of 2

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s REFRENCES: 1.Mahadevan M, Trounson AO. Effect of cooling, freezing and thawing rates and storage conditions on preservation of human spermatozoa. Andrologia 1984 ;16:1.52-60.

2.Ozkavukcu S, Erdemli E, Isik A, Oztuna D, Karahusevinoglu S. Effects of cryopreservation on sperm parameters and ultrastructural morphology of human spermatozoa. J Assist Reorod Genet 2008; 8: 403-11. [OpenAIRE]

3.Yavin S, Arav A. Measurement of essential physical properties of vitrification solutions.

Theriogenology 2007; 1: 81-9.

4.Rall WF, Fahy GM. Ice-free cryopreservation of mouse embryos at -196ºC by vitrification.

Nature 1985; 313: 573-5.

5.Isachenko E, Isachenko V, Katkov II, Nawroth F. Vitrification of human spermatozoa without cryoprotectants: review of problem and practical success. RBMonline 2003; 6: 191-200.

6.Ramos L, Wetzels AM. Low rates of DNA fragmentation in selected motile human spermatozoa assessed by the TUNEL assay. Hum Reprod 2001; 8: 1703-7. [OpenAIRE]

7.World Health Organization. WHO laboratory manual for the examination of human semen and sperm-cervical mucus interaction. Cambridge University Press: New York: 1999; 4-34.

8.Brotherton J. Cryopreservation of human semen. Arch Androl 1990; 25:181-95.

9.Isachenko V, Isachenko E, Montag M, Zaeva V, Krivokharchenko A, Nawroth F et al. Clean technique for cryoprotectant - free vitrification of human spermatozoa. Reprod Biomed 2005; 10(3): 350-4.

10.Dalzell LH, Thompson -Cree ME, Mc Clure N, Traub AI. Lewis SE Effects of 24 hour incubation after freeze-thawing on DNA fragmentation of testicular sperm from infertile and fertile men. Fertil Steril 2003, 79: 1670-2.

25 references, page 1 of 2
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