publication . Article . 2003

Mapping the proteome of Leishmania Viannia parasites using two-dimensional polyacrylamide gel electrophoresis and associated technologies

Góngora R; Acestor N; Quadroni M; Nicolas Fasel; Ng, Saravia; Walker J;
  • Published: 01 Jun 2003
Abstract
In this study we have demonstrated the potential of two-dimensional electrophoresis (2DE)-based technologies as tools for characterization of the Leishmania proteome (the expressed protein complement of the genome). Standardized neutral range (pH 5-7) proteome maps of Leishmania (Viannia) guyanensis and Leishmania (Viannia) panamensis promastigotes were reproducibly generated by 2DE of soluble parasite extracts, which were prepared using lysis buffer containing urea and nonidet P-40 detergent. The Coomassie blue and silver nitrate staining systems both yielded good resolution and representation of protein spots, enabling the detection of approximately 800 and 1,...
Subjects
Medical Subject Headings: parasitic diseases
free text keywords: proteomics, proteome, leishmania, two-dimensional polyacrylamide gel electrophoresis, Medicine, R, Arctic medicine. Tropical medicine, RC955-962, Lysis buffer, Ribosomal protein, Polyacrylamide gel electrophoresis, biology.organism_classification, biology, Membrane protein, Molecular biology, Kinetoplast
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publication . Article . 2003

Mapping the proteome of Leishmania Viannia parasites using two-dimensional polyacrylamide gel electrophoresis and associated technologies

Góngora R; Acestor N; Quadroni M; Nicolas Fasel; Ng, Saravia; Walker J;