Application of PCR-based DNA sequencing technique for the detection of Leptospira in peripheral blood of septicemia patients

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Ram, S. ; Vimalin, J.M. ; Jambulingam, M. ; Tiru, V. ; Gopalakrishnan, R.K. ; Madhavan, H.N. (2012)
  • Publisher: Malaysian Society for Microbiology
  • Journal: Malaysian Journal of Microbiology (issn: 1823-8262, eissn: 2231-7538)
  • Subject: DNA sequencing | LipL32 gene | Microbiology | QR1-502 | Leptospira | Polymerase chain reaction
    mesheuropmc: bacterial infections and mycoses | animal diseases

Aim: Isolation, dark field detection and microscopic agglutination test (MAT) are considered ―gold standard‖ tests for diagnosis of Leptospirosis. Several PCR assays are reported but very few have been evaluated for detection of Leptospirosis. Therefore, this study was ... View more
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