publication . Article . 2018

Expression of Pinellia pedatisecta Lectin Gene in Transgenic Wheat Enhances Resistance to Wheat Aphids

Xiaoliang Duan; Qiling Hou; Guoyu Liu; Xiaomeng Pang; Zhenli Niu; Xiao Wang; Yufeng Zhang; Baoyun Li; Rongqi Liang;
Open Access English
  • Published: 01 Mar 2018 Journal: Molecules : A Journal of Synthetic Chemistry and Natural Product Chemistry, volume 23, issue 4 (eissn: 1420-3049, Copyright policy)
  • Publisher: MDPI
Abstract
Wheat aphids are major pests during the seed filling stage of wheat. Plant lectins are toxic to sap-sucking pests such as wheat aphids. In this study, Pinellia pedatisecta agglutinin (ppa), a gene encoding mannose binding lectin, was cloned, and it shared 92.69% nucleotide similarity and 94% amino acid similarity with Pinellia ternata agglutinin (pta). The ppa gene, driven by the constitutive and phloem-specific ribulose bisphosphate carboxylase small subunit gene (rbcs) promoter in pBAC-rbcs-ppa expression vector, was transferred into the wheat cultivar Baofeng104 (BF104) by particle bombardment transformation. Fifty-four T0 transgenic plants were generated. Th...
Subjects
Medical Subject Headings: food and beveragesrespiratory systembiochemical phenomena, metabolism, and nutrition
free text keywords: Article, Pinellia pedastisecta, ppa, lectin, wheat aphid, aphid resistance, Organic chemistry, QD241-441
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publication . Article . 2018

Expression of Pinellia pedatisecta Lectin Gene in Transgenic Wheat Enhances Resistance to Wheat Aphids

Xiaoliang Duan; Qiling Hou; Guoyu Liu; Xiaomeng Pang; Zhenli Niu; Xiao Wang; Yufeng Zhang; Baoyun Li; Rongqi Liang;