publication . Article . 2014

A Comparison between the Cytotoxicity Induced by Gossypol in Two Testicular Cell Lines

Neda MahdinezhadGorji; SeyedGholamAli Jorsaraei; Vida Hojati; Ebrahim Zabihi; Asieh Khalilpour; Zainab Abedian; Eisa Tahmasbpour;
Open Access English
  • Published: 01 Dec 2014 Journal: Iranian Journal of Toxicology (issn: 2008-2967, eissn: 2251-9459, Copyright policy)
  • Publisher: Arak University of Medical Sciences
Abstract
Background: Gossypol is a yellow toxic pigment from the cottonseed that can cause acute or chronic toxicity in humans and animals by affecting the testicular tissues. Nowadays cottonseed is used as food supplement for ruminants specially the sheep. In this study, two different stem cell lines of testicular tissue including GC1-spg (mouse testis) and SFTF-PI43 (sheep testis) cells were used to evaluation of gossypol cytotoxicity. Methods: The GC-1spg and the SFTF_PI43 cells were cultured in RPMI-1640 supplemented with fetal bovine serum (10%) and antibiotic (penicillin 105/ml, streptomycin100μg/ml), and then 5×104 cells/well were seeded in 24 well plates. Culture...
Subjects
free text keywords: Cytotoxicity, GC1-Spg, Germ Cell Line, SFTF-PI43, Testis., Toxicology. Poisons, RA1190-1270
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