publication . Article . 2010

Protección preferencial de los dominios II y III de la toxina Cry1Aa de Bacillus thuringiensis en Vesículas de Membrana de Borde de Cepillo

Syed-Rehan A., Hussain; Flórez, Álvaro M.; Donald H, Dean; Alzate, Óscar;
Open Access Spanish
  • Published: 01 Dec 2010 Journal: Revista Colombiana de Biotecnología (issn: 0123-3475, eissn: 1909-8758, Copyright policy)
  • Publisher: Universidad Nacional de Colombia
Abstract
Título español: Protección preferencial de los dominios II y III de la toxina Cry1Aa de Bacillus thuringiensis en Vesículas de Membrana de Borde de Cepillo Abstract The surface exposed Leucine 371 on loop 2 of domain II, in Cry1Aa toxin, was mutated to Lysine to generate the trypsin-sensitive mutant, L371K. Upon trypsin digestion L371K is cleaved into approximately 37 and 26 kDa fragments. These are separable on SDS-PAGE, but remain as a single molecule of 65 kDa upon purification by liquid chromatography. The larger fragment is domain I and a portion of domain II (amino acid residues 1 to 371). The smaller 26-kDa polypeptide is the remainder of domain II and do...
Subjects
Medical Subject Headings: fungi
free text keywords: Ingeniería y Tecnología, Ingeniería Química, Ingeniería de Procesos, e -endotoxin, δ -endotoxin, δendotoxina, Bacillus thuringiensis, site directed mutagenesis,  -endotoxin, mutagénesis sitio dirigida,  - endotoxina, Biotechnology, TP248.13-248.65, mutagénesis sitio dirigida,  - endotoxina
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publication . Article . 2010

Protección preferencial de los dominios II y III de la toxina Cry1Aa de Bacillus thuringiensis en Vesículas de Membrana de Borde de Cepillo

Syed-Rehan A., Hussain; Flórez, Álvaro M.; Donald H, Dean; Alzate, Óscar;