publication . Article . 2014

Borrelia burgdorferi genospecies detection by RLB hybridization in Ixodes cinus ticks from different sites of North-Eastern Poland

Justyna Dunaj; Joanna Maria Zajkowska; Maciej Kondrusik; Lise Gern; Oliver Rais; Anna Moniuszko; Sławomir Pancewicz; Renata Świerzbińska;
Open Access English
  • Published: 01 Jun 2014 Journal: Annals of Agricultural and Environmental Medicine, volume 21, issue 874,132, pages 239-243 (issn: 1232-1966, eissn: 1898-2263, Copyright policy)
  • Publisher: Institute of Rural Health
Abstract
Introduction. RLB (Reverse Line Blot Hybridization) is a molecular biology technique that might be used for [i]Borrelia burgdorferi [/i]sensu lato (sl) DNA detection with genospecies specification. Among[i] B. burgdorferi[/i] sl genospecies at least 7 are regarded as pathogenic in Europe. objective. The aim of the study was to evaluate the frequency of different [i]Borrelia[/i] genospecies DNA detection in Ixodes ricinus ticks in the endemic area of North-Eastern Poland by using RLB. materials and method. Ixodes ricinus ticks were collected in May – June, from 6 different sites in North-Eastern Poland (Jakubin, Kolno, Grajewo, Suwałki, Siemiatycze, Białowieża) b...
Subjects
Medical Subject Headings: bacterial infections and mycosesparasitic diseases
free text keywords: 5S 23S intergenic spacer;genospecies-identification;Borrelia burgdorferi;Ixodes ricinus ticks;reverse line blotting, lcsh:Agriculture, lcsh:S, lcsh:Environmental sciences, lcsh:GE1-350
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