publication . Article . 2011

Real-time PCR quantification and diversity analysis of the functional genes aprA and dsrA of sulfate-reducing prokaryotes in marine sediments of the Peru continental margin and the Black Sea

Axel Schippers; Anna Blazejak;
Open Access English
  • Published: 01 Dec 2011
  • Publisher: Lausanne : Frontiers Research Foundation
  • Country: Germany
Abstract
Sulfate-reducing prokaryotes (SRP) are ubiquitous and quantitatively important members in many ecosystems, especially in marine sediments. However their abundance and diversity in subsurface marine sediments is poorly understood. In this study, the abundance and diversity of the functional genes for the enzymes adenosine 5'-phosphosulfate reductase (aprA) and dissimilatory sulfite reductase (dsrA) of SRP in marine sediments of the Peru continental margin and the Black Sea were analyzed, including samples from the deep biosphere (ODP site 1227). For aprA quantification a Q-PCR assay was designed and evaluated. Depth profiles of the aprA and dsrA copy numbers were...
Subjects
free text keywords: deep biosphere, real-time pcr, subsurface, odp, sulfate-reducing prokaryotes, apra, dsra, Microbiology, Original Research, QR1-502, Microbiology (medical), ddc:500, Sulfate, chemistry.chemical_compound, chemistry, Gene, Extreme environment, Mbsf, Bacteria, biology.organism_classification, biology, Ecology, Sediment, 16S ribosomal RNA, clone (Java method)
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