Detection of Candida albicans Sap2 in cancer patient serum samples by an indirect competitive enzyme-linked immunosorbent assay for the diagnosis of candidiasis

Article English OPEN
Yicun Wang ; Xiang Gao ; J U Zhi gang ; Jingyuan Liu ; Shuai Dong ; Li Wang (2013)
  • Publisher: Wolters Kluwer Medknow Publications
  • Journal: Indian Journal of Pathology and Microbiology (issn: 0377-4929)
  • Related identifiers: doi: 10.4103/0377-4929.120377
  • Subject: Antibody | cancer | Candida albicans | ELISA | Sap2 | Pathology | RB1-214 | Microbiology | QR1-502

Background: The secreted aspartyl proteinases 2 (Sap2) of Candida albicans (C. albicans) is a potential marker of candididasis. It is a virulence factor associated with adherence and tissue invasion. Aim: In order to detect Sap2 in clinical sera, we developed an indirect competitive enzyme-linked immunosorbent assay (ELISA). Materials and Methods: Polyclonal antibodies were produced for Sap2 by injecting Sap2 into a New Zealand White inbred rabbit. They could be used at a dilution exceeding 1:1200 in an indirect ELISA, and detected Sap2 concentration up to 1 ng/mL. Results: Of the 286 cancer serum samples tested, 16.8% were found as candidiasis. The test was simple and economical to perform and had a level of sensitivity for detection of low-titer positive sera; thus, it may be proven to be of value in epidemiological studies on candidiasis.
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