publication . Article . 2013

Detection of Candida albicans Sap2 in cancer patient serum samples by an indirect competitive enzyme-linked immunosorbent assay for the diagnosis of candidiasis

Li Wang; Yicun Wang; Xiang Gao; JU Zhi gang; Jingyuan Liu; Shuai Dong;
Open Access English
  • Published: 01 Jan 2013 Journal: Indian Journal of Pathology and Microbiology (issn: 0377-4929, Copyright policy)
  • Publisher: Wolters Kluwer Medknow Publications
Abstract
Background: The secreted aspartyl proteinases 2 (Sap2) of Candida albicans ( C. albicans ) is a potential marker of candididasis. It is a virulence factor associated with adherence and tissue invasion. Aim: In order to detect Sap2 in clinical sera, we developed an indirect competitive enzyme-linked immunosorbent assay (ELISA). Materials and Methods: Polyclonal antibodies were produced for Sap2 by injecting Sap2 into a New Zealand White inbred rabbit. They could be used at a dilution exceeding 1:1200 in an indirect ELISA, and detected Sap2 concentration up to 1 ng/mL. Results: Of the 286 cancer serum samples tested, 16.8% were found as candidiasis. The test was s...
Subjects
free text keywords: Antibody, cancer, Candida albicans, ELISA, Sap2, Pathology, RB1-214, Microbiology, QR1-502, Pathology and Forensic Medicine, Microbiology (medical), General Medicine, medicine.disease, medicine, Virulence factor, Enzyme, chemistry.chemical_classification, chemistry, Polyclonal antibodies, biology.protein, biology, biology.organism_classification, Aspartyl Proteinases, medicine.medical_specialty, business.industry, business, Immunology, Corpus albicans
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publication . Article . 2013

Detection of Candida albicans Sap2 in cancer patient serum samples by an indirect competitive enzyme-linked immunosorbent assay for the diagnosis of candidiasis

Li Wang; Yicun Wang; Xiang Gao; JU Zhi gang; Jingyuan Liu; Shuai Dong;