publication . Article . 2017

Immobilized Lipases on Functionalized Silica Particles as Potential Biocatalysts for the Synthesis of Fructose Oleate in an Organic Solvent/Water System

Vescovi, Vinicius; Giordano, Raquel L. C.; Mendes, Adriano A.; Tardioli, Paulo W.;
Open Access English
  • Published: 30 Jan 2017 Journal: Molecules (issn: 1420-3049, Copyright policy)
  • Publisher: MDPI AG
Abstract
Lipases from Thermomyces lanuginosus (TLL) and Pseudomonas fluorescens (PFL) were immobilized on functionalized silica particles aiming their use in the synthesis of fructose oleate in a tert-butyl alcohol/water system. Silica particles were chemically modified with octyl (OS), octyl plus glutaraldehyde (OSGlu), octyl plus glyoxyl (OSGlx), and octyl plus epoxy groups (OSEpx). PFL was hyperactivated on all functionalized supports (more than 100% recovered activity) using low protein loading (1 mg/g), however, for TLL, this phenomenon was observed only using octyl-silica (OS). All prepared biocatalysts exhibited high stability by incubating in tert-butyl alcohol (...
Subjects
free text keywords: microbial  lipases,   immobilization,   functionalized  silica  particles,   fructose  oleate  synthesis,  organic solvent/water system, Organic chemistry, QD241-441, Article, microbial lipases, immobilization, functionalized silica particles, fructose oleate synthesis, organic solvent/water system, Pseudomonas fluorescens, biology.organism_classification, biology, Catalysis, Chemistry, Enzyme, chemistry.chemical_classification, Biochemistry, Fructose, chemistry.chemical_compound, Glutaraldehyde, Solvent, Alcohol, Operational stability
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publication . Article . 2017

Immobilized Lipases on Functionalized Silica Particles as Potential Biocatalysts for the Synthesis of Fructose Oleate in an Organic Solvent/Water System

Vescovi, Vinicius; Giordano, Raquel L. C.; Mendes, Adriano A.; Tardioli, Paulo W.;