Work alienation, its causes and effects are among the key issues in the organizational and management sciences. This study was conducted to investigate the effect of job characteristics and perceived organizational support on work alienation. All the expert personnel of a governmental company in an electrical industry located in Shiraz were the population of this study out of whom 131 were randomly selected to complete the standard questionnaire. The present study is a descriptive-correlational study. The Data analysis was done via SEM using Smart PLS 2.0. The results showed that organizational support and job characteristics have a significant negative effect on work alienation. The job characteristic variable has a higher explanatory power in the changes occurring in work alienation variable