Efficient and Cost-Effective Generation of Mature Neurons From Human Induced Pluripotent Stem Cells

Article, Other literature type English OPEN
Badja, Cherif; Maleeva, Galyna; El-Yazidi, Claire; Barruet, Emilie; Lasserre, Manon; Tropel, Philippe; Binetruy, Bernard; Bregestovski, Piotr; Magdinier, Frédérique;
(2014)
  • Publisher: AlphaMed Press
  • Related identifiers: doi: 10.5966/sctm.2014-0024, pmc: PMC4250204
  • Subject: [ SDV.GEN.GH ] Life Sciences [q-bio]/Genetics/Human genetics | Enabling Technologies for Cell-Based Clinical Translation
    mesheuropmc: nervous system

The authors describe a feeder-free method of generating induced pluripotent stem cells by relying on the use of a chemically defined medium that overcomes the need for embryoid body formation and neuronal rosette isolation for neuronal precursors and terminally differen... View more
  • References (25)
    25 references, page 1 of 3

    1 Takahashi K, Yamanaka S. Induction of pluripotent stem cells from mouse embryonic and adult fibroblast cultures by defined factors. Cell 2006;126:663-676.

    2 Park IH, Zhao R, West JA et al. Reprogramming of human somatic cells to pluripotency with defined factors. Nature 2008;451:141- 146.

    3 Lee G, Papapetrou EP, Kim H et al. Modelling pathogenesis and treatment of familial dysautonomia using patient-specific iPSCs. Nature 2009;461:402-406.

    4 Brennand KJ, Simone A, Jou J et al. Modelling schizophrenia using human induced pluripotent stem cells. Nature 2011;473:221- 225.

    5 Chamberlain SJ, Li XJ, Lalande M. Induced pluripotent stem (iPS) cells as in vitro models of human neurogenetic disorders. Neurogenetics 2008;9:227-235.

    6 Vigano` F, Mo¨bius W, Go¨tz M et al. Transplantation reveals regional differences in oligodendrocyte differentiation in the adult brain. Nat Neurosci 2013;16:1370-1372.

    7 Espuny-Camacho I, Michelsen KA, Gall D et al. Pyramidal neurons derived from human pluripotent stem cells integrate efficiently into mouse brain circuits in vivo. Neuron 2013;77: 440-456.

    8 Yang N, Ng YH, Pang ZP et al. Induced neuronal cells: How to make and define a neuron. Cell Stem Cell 2011;9:517-525.

    9 Carpenter M, Rao MS, Freed W et al. Derivation and characterization of neuronal precursors and dopaminergic neurons from human embryonic stem cells in vitro. Methods Mol Biol 2006;331:153-167.

    10 Fucile S, De Saint Jan D, de Carvalho LP et al. Fast potentiation of glycine receptor channels of intracellular calcium in neurons and transfected cells. Neuron 2000;28:571- 583.

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