An Extended Multilocus Sequence Typing (MLST) Scheme for Rapid Direct Typing of Leptospira from Clinical Samples.

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Weiss, Sabrina; Menezes, Angela; Woods, Kate; Chanthongthip, Anisone; Dittrich, Sabine; Opoku-Boateng, Agatha; Kimuli, Maimuna; Chalker, Victoria;
(2016)
  • Publisher: Public Library of Science (PLoS)
  • Journal: PLoS Neglected Tropical Diseases,volume 10,issue 9 (issn: 1935-2727, eissn: 1935-2735)
  • Publisher copyright policies & self-archiving
  • Related identifiers: doi: 10.1371/journal.pntd.0004996, pmc: PMC5031427
  • Subject: Molecular Biology | Microbial Pathogens | Leptospirosis | Research Article | Molecular Biology Assays and Analysis Techniques | Bacteria | Anatomy | Urine | Infectious Diseases | Pathology and Laboratory Medicine | Leptospira | Genetic Loci | Artificial Gene Amplification and Extension | Phylogenetic Analysis | Neglected Tropical Diseases | Genetics | Pathogens | Molecular Biology Techniques | Polymerase Chain Reaction | Biology and Life Sciences | Research and Analysis Methods | Microbiology | Physiology | Zoonoses | Bacterial Pathogens | Body Fluids | Tropical Diseases | Bacterial Diseases | Medical Microbiology | Leptospira Interrogans | RC955-962 | RA1-1270 | Public aspects of medicine | Organisms | Medicine and Health Sciences | Arctic medicine. Tropical medicine

Background Rapid typing of Leptospira is currently impaired by requiring time consuming culture of leptospires. The objective of this study was to develop an assay that provides multilocus sequence typing (MLST) data direct from patient specimens while minimising costs ... View more
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