MEDIATOR18 and MEDIATOR20 confer susceptibility to Fusarium oxysporum in Arabidopsis thaliana.

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Thorya Fallath ; Brendan N Kidd ; Jiri Stiller ; Celine Davoine ; Stefan Björklund ; John M Manners ; Kemal Kazan ; Peer M Schenk

Publisher: Public Library of Science (PLoS)
Journal: PLoS ONE, volume 12, issue 4 (issn: 1932-6203, eissn: 1932-6203)
Related identifiers: doi: 10.1371/journal.pone.0176022, pmc: PMC5404846
Subject: Molecular biology | Signaling Cascades | Research Article | Transcription Factors | Regulatory Proteins | Genetics | Leaves | Flowering Plants | Stress Signaling Cascade | Plants | Molecular biology techniques | Proteins | Plant Science | Experimental Organism Systems | Medical Biotechnology (with a focus on Cell Biology (including Stem Cell Biology), Molecular Biology, Microbiology, Biochemistry or Biopharmacy) | Sequencing techniques | DNA-binding proteins | Biology and Life Sciences | Signal Transduction | Research and analysis methods | Plant Anatomy | Medicinsk bioteknologi (med inriktning mot cellbiologi (inklusive stamcellsbiologi), molekylärbiologi, mikrobiologi, biokemi eller biofarmaci) | Medicine | Plant and Algal Models | RNA sequencing | Q | R | Cell Biology | Phenotypes | Model Organisms | Gene Regulation | Cell Signaling | Biochemistry | Science | Organisms | Brassica | Arabidopsis Thaliana | Gene Expression

The conserved protein complex known as Mediator conveys transcriptional signals by acting as an intermediary between transcription factors and RNA polymerase II. As a result, Mediator subunits play multiple roles in regulating developmental as well as abiotic and biotic stress pathways. In this report we identify the head domain subunits MEDIATOR18 and MEDIATOR20 as important susceptibility factors for Fusarium oxysporum infection in Arabidopsis thaliana. Mutants of MED18 and MED20 display down-regulation of genes associated with jasmonate signaling and biosynthesis while up-regulation of salicylic acid associated pathogenesis related genes and reactive oxygen producing and scavenging genes. We propose that MED18 and MED20 form a sub-domain within Mediator that controls the balance of salicylic acid and jasmonate associated defense pathways.

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Related Research Results (9)

Inferred

Network Properties of Robust Immunity in Plants	73%
Investigating the Association between Flowering Time and Defense in the <i>Arabidopsis thaliana-Fusarium oxysporum</i> Interaction	73%
<em>Mediator Subunit18</em> Controls Flowering Time and Floral Organ Identity in <em>Arabidopsis</em>	73%
Ethylene Response Factor 6 Is a Regulator of Reactive Oxygen Species Signaling in <i>Arabidopsis</i>	73%
<i>Fusarium oxysporum</i> Triggers Tissue-Specific Transcriptional Reprogramming in <i>Arabidopsis thaliana</i>	73%
The expression of plant defense and JA-associated genes is increased in the WT relative to <i>med18</i> and <i>med20</i> in response to <i>F</i>. <i>oxysporum</i> infection.	73%
<i>Pseudomonas syringae</i> pv. <i>syringae</i> Uses Proteasome Inhibitor Syringolin A to Colonize from Wound Infection Sites	73%
<i>MEDIATOR18</i> and <i>MEDIATOR20</i> confer susceptibility to <i>Fusarium oxysporum</i> in <i>Arabidopsis thaliana</i>	73%
The Arabidopsis KH-Domain RNA-Binding Protein ESR1 Functions in Components of Jasmonate Signalling, Unlinking Growth Restraint and Resistance to Stress	73%

Similar Research Results (6)

Structure–system correlation identifies a gene regulatory Mediator submodule. (2008)	85%
The head module of Mediator directs activation of preloaded RNAPII in vivo (2013)	82%
Med8, Med18, and Med20 subunits of the Mediator head domain are interdependent upon each other for folding and complex formation (2009)	81%
Structure and TBP binding of the Mediator head subcomplex Med8–Med18–Med20. (2006)	80%
Mediator regulates non-coding RNA transcription at fission yeast centromeres (2012)	77%
Two conserved modules of Schizosaccharomyces pombe Mediator regulate distinct cellular pathways (2008)	77%

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