publication . Article . Other literature type . 1997

Mobility of creatine phosphokinase and beta-enolase in cultured muscle cells

Arrio-Dupont, M; Foucault, G; Vacher, M; Douhou, A; Cribier, S;
Open Access English
  • Published: 01 Nov 1997 Journal: Biophysical Journal, volume 73, issue 5, pages 2,667-2,673 (issn: 0006-3495, Copyright policy)
  • Publisher: Elsevier BV
Abstract
The diffusion of beta-enolase and creatine phosphokinase in muscle cells has been studied by modulated fringe pattern photobleaching. Beta-enolase is mobile in the sarcoplasm. At 20 degrees C, the diffusion coefficient is 13.5 +/- 2.5 microm2 s(-1) in the cytosol and 56 microm2 s(-1) in aqueous media. As in the case of dextrans of the same hydrodynamic radius, its mobility is hindered by both the crowding of the fluid phase of the cytoplasm and the screening effect due to myofilaments. A fraction of creatine phosphokinase is mobile in the sarcoplasm. Its diffusion coefficient in the cytosol, 4.5 +/- 1 microm2 s(-1), is lower than that of the dextran of equivalen...
Subjects
free text keywords: Research Article, Biophysics
Powered by OpenAIRE Open Research Graph
Any information missing or wrong?Report an Issue
publication . Article . Other literature type . 1997

Mobility of creatine phosphokinase and beta-enolase in cultured muscle cells

Arrio-Dupont, M; Foucault, G; Vacher, M; Douhou, A; Cribier, S;