Comparison of Real-Time Quantitative PCR and Culture for the Diagnosis of Emerging Rickettsioses

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Emmanouil Angelakis; Hervé Richet; Jean-Marc Rolain; Bernard La Scola; Didier Raoult;
  • Publisher: Public Library of Science
  • Journal: PLoS Neglected Tropical Diseases,volume 6,issue 3 (issn: 1935-2727, eissn: 1935-2735)
  • Publisher copyright policies & self-archiving
  • Related identifiers: doi: 10.1371/journal.pntd.0001540, pmc: PMC3295807
  • Subject: Diagnostic medicine | Research Article | Skin infections | Infectious Diseases | Health Care Policy | [SDV.MHEP.MI]Life Sciences [q-bio]/Human health and pathology/Infectious diseases | Epidemiology | Serology | [ SDV.MHEP.MI ] Life Sciences [q-bio]/Human health and pathology/Infectious diseases | Vectors and Hosts | Polymerase chain reaction | Non-Clinical Medicine | Rickettsia | Biology | Clinical Research Design | Microbiology | Medicine | Specimen preparation and treatment | Bacterial Diseases | RC955-962 | Dermatology | RA1-1270 | Drugs and Devices | Public aspects of medicine | Biopsy | Arctic medicine. Tropical medicine | Antibiotics
    mesheuropmc: bacterial infections and mycoses

BACKGROUND: Isolation of Rickettsia species from skin biopsies may be replaced by PCR. We evaluated culture sensitivity compared to PCR based on sampling delay and previous antibiotic treatment. METHODOLOGY/PRINCIPAL FINDINGS: Skin biopsies and ticks from patients with ... View more
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