publication . Article . 2012

Relation of genetic variants of CYP2A6 with tobacco dependence and smoking habit in Chilean subjects: A pilot study

Dante D Cáceres; Sergio A Alvarado; Paulina Martínez; Luis A Quiñones;
Open Access Spanish
  • Published: 01 Apr 2012
  • Publisher: Sociedad Médica de Santiago
Background: Genetic and metabolic factors associated with nicotine metabolism may be related to smoking behavior. Aim: To assess the prevalence of allelic and genotype variants of CYP2A6 in a sample of Chilean subjects and to evaluate their relationship with smoking and tobacco dependence. Material and Methods: The genotype frequencies for *2, *3 and *4 of CYP2A6*1 (wild type) gene were determined by polymerase chain reaction (PCR) in 54 volunteers. Addiction to tobacco was evaluated using the Fagerstrom Test. The association between the presence of allelic variants of CYP2A6 and smoking and tobacco dependence was evaluated with chi square test. Results: The pre...
free text keywords: CYP2A6, Genetic, Polymorphisms, Protein, human, Tobacco use disorder, General Medicine, Wild type, Endocrinology, medicine.medical_specialty, medicine, business.industry, business, Chi-square test, Internal medicine, Allele, Genotype frequency, Diabetes mellitus, medicine.disease, Genotype, Polymerase chain reaction, law.invention, law
33 references, page 1 of 3

1. Nebot M, Puig R, Ballestin M, Albericci M. [Tobacco in Barcelona's metropolitan transportation: observation study]. Aten Primaria 2001; 28 (1): 50-2.

2. Bleger J. Psicología de la Conducta. 1998.

3. Walton R, Johnstone E, Munafo M, Neville M, Griffiths S. Genetic clues to the molecular basis of tobacco addiction and progress towards personalized therapy. Trends Mol Med 2001; 7 (2): 70-6.

4. Munafo M, Johnstone E, Murphy M, Walton R. New directions in the genetic mechanisms underlying nicotine addiction. Addict Biol 2001; 6 (2): 109-17.

5. Hamdani N, Ades J, Gorwood P. [Heritability and candidate genes in tobacco use]. Encephale 2006; 32 (6 Pt 1): 966-75.

6. Madden PA, Heath AC, Pedersen NL, Kaprio J, Koskenvuo MJ, Martin NG. The genetics of smoking persistence in men and women: a multicultural study. Behav Genet 1999; 29 (6): 423-31.

7. Nakajima M, Yokoi T. Interindividual variability in nicotine metabolism: C-oxidation and glucuronidation. Drug Metab Pharmacokinet 2005; 20 (4): 227-35.

8. Hukkanen J, Jacob P, 3rd, Benowitz NL. Metabolism and disposition kinetics of nicotine. Pharmacol Rev 2005; 57 (1): 79-115.

9. Céspedes-Lantigua L, Lorenzo Rodríguez A, CastañerHerrera J, Pérez-Coronel P. Consideraciones y papel del médico de familia en la desestimulación del hábito de fumar. Rev Cubana Med Gen Integr 2001; 17 (6): 606- 10.

10. Fernández-Salguero P, Hoffman SM, Cholerton S, Mohrenweiser H, Raunio H, Rautio A, et al. A genetic polymorphism in coumarin 7-hydroxylation: sequence of the human CYP2A genes and identification of variant CYP2A6 alleles. Am J Hum Genet 1995; 57 (3): 651-60.

11. Lessov-Schlaggar CN, Pergadia ML, Khroyan TV, Swan GE. Genetics of nicotine dependence and pharmacotherapy. Biochem Pharmacol 2008; 75 (1): 178-95.

12. Inoue K, Yamazaki H, Shimada T. CYP2A6 genetic polymorphisms and liver microsomal coumarin and nicotine oxidation activities in Japanese and Caucasians. Arch Toxicol 2000; 73 (10-11): 532-9.

13. Nakajima M, Yamagishi S, Yamamoto H, Yamamoto T, Kuroiwa Y, Yokoi T. Deficient cotinine formation from nicotine is attributed to the whole deletion of the CYP2A6 gene in humans. Clin Pharmacol Ther 2000; 67 (1): 57-69.

14. Kitagawa K, Kunugita N, Katoh T, Yang M, Kawamoto T. The significance of the homozygous CYP2A6 deletion on nicotine metabolism: a new genotyping method of CYP2A6 using a single PCR-RFLP. Biochem Biophys Res Commun 1999; 262 (1): 146-51.

15. Murphy SE, Johnson LM, Pullo DA. Characterization of multiple products of cytochrome P450 2A6-catalyzed cotinine metabolism. Chem Res Toxicol 1999; 12 (7): 639-45.

33 references, page 1 of 3
Powered by OpenAIRE Research Graph
Any information missing or wrong?Report an Issue