Thermophilic bacteria in Moroccan hot springs, salt marshes and desert soils

Article English OPEN
Aanniz, Tarik ; Ouadghiri, Mouna ; Melloul, Marouane ; Swings, Jean ; Elfahime, Elmostafa ; Ibijbijen, Jamal ; Ismaili, Mohamed ; Amar, Mohamed (2015)
  • Publisher: Sociedade Brasileira de Microbiologia
  • Journal: Brazilian Journal of Microbiology, volume 46, issue 2, pages 443-453 (issn: 1517-8382, eissn: 1678-4405)
  • Related identifiers: pmc: PMC4507536, doi: 10.1590/S1517-838246220140219
  • Subject: diversity | Environmental Microbiology | thermophilic bacteria | hot springs | Microbiology | hydrolytic enzymes | QR1-502 | 16S rRNA gene sequencing

The diversity of thermophilic bacteria was investigated in four hot springs, three salt marshes and 12 desert sites in Morocco. Two hundred and forty (240) thermophilic bacteria were recovered, identified and characterized. All isolates were Gram positive, rod-shaped, spore forming and halotolerant. Based on BOXA1R-PCR and 16S rRNA gene sequencing, the recovered isolates were dominated by the genus Bacillus (97.5%) represented by B. licheniformis (119), B. aerius (44), B. sonorensis (33), B. subtilis (subsp. spizizenii (2) and subsp. inaquosurum (6)), B. amyloliquefaciens (subsp. amyloliquefaciens (4) and subsp. plantarum (4)), B. tequilensis (3), B. pumilus (3) and Bacillus sp. (19). Only six isolates (2.5%) belonged to the genus Aeribacillus represented by A. pallidus (4) and Aeribacillus sp. (2). In this study, B. aerius and B. tequilensis are described for the first time as thermophilic bacteria. Moreover, 71.25%, 50.41% and 5.41% of total strains exhibited high amylolytic, proteolytic or cellulolytic activity respectively.