publication . Article . Other literature type . 2011

OSBP-Related Protein 8 (ORP8) Regulates Plasma and Liver Tissue Lipid Levels and Interacts with the Nucleoporin Nup62

Tianhong Zhou; Shiqian Li; Wenbin Zhong; Terhi Vihervaara; Olivier Béaslas; Julia Perttilä; Wei Luo; Yingliang Jiang; Markku Lehto; Vesa M. Olkkonen; ...
Open Access English
  • Published: 15 Jun 2011 Journal: PLoS ONE, volume 6, issue 6 (eissn: 1932-6203, Copyright policy)
  • Publisher: Public Library of Science
  • Country: Finland
Abstract
We earlier identified OSBP-related protein 8 (ORP8) as an endoplasmic reticulum oxysterol-binding protein implicated in cellular lipid homeostasis. We now investigated its action in hepatic cells in vivo and in vitro. Adenoviral overexpression of ORP8 in mouse liver induced a decrease of cholesterol, phospholipids, and triglycerides in serum (-34%, -26%, -37%, respectively) and liver tissue (-40%, -12%, -24%), coinciding with reduction of nuclear (n)SREBP-1 and -2 and mRNA levels of their target genes. Consistently, excess ORP8 reduced nSREBPs in HuH7 cells, and ORP8 overexpression or silencing by RNA interference moderately suppressed or induced the expression ...
Subjects
Medical Subject Headings: lipids (amino acids, peptides, and proteins)
free text keywords: Research Article, Biology, Model Organisms, Animal Models, Mouse, Molecular cell biology, Cellular Structures, Cell Nucleus, Gene expression, RNA interference, Membranes and Sorting, Medicine, R, Science, Q, 3111 Biomedicine, General Biochemistry, Genetics and Molecular Biology, General Agricultural and Biological Sciences, General Medicine, Molecular biology, Bimolecular fluorescence complementation, Hepatic stellate cell, Endoplasmic reticulum, Lipid metabolism, Sterol regulatory element-binding protein, Transfection, Nucleoporin
Related Organizations
Funded by
AKA| Cytoplasmic sterol sensors for integrated control of cellular lipid metabolism
Project
  • Funder: Academy of Finland (AKA)
  • Project Code: 121457
,
EC| LIPIDOMICNET
Project
LIPIDOMICNET
Lipid droplets as dynamic organelles of fat deposition and release: Translational research towards human disease
  • Funder: European Commission (EC)
  • Project Code: 202272
  • Funding stream: FP7 | SP1 | HEALTH
51 references, page 1 of 4

Horton, JD, Goldstein, JL, Brown, MS. SREBPs: activators of the complete program of cholesterol and fatty acid synthesis in the liver.. J Clin Invest. 2002; 109: 1125-1131 [OpenAIRE] [PubMed]

Goldstein, JL, DeBose-Boyd, RA, Brown, MS. Protein sensors for membrane sterols.. Cell. 2006; 124: 35-46 [OpenAIRE] [PubMed]

Eberle, D, Hegarty, B, Bossard, P, Ferre, P, Foufelle, F. SREBP transcription factors: master regulators of lipid homeostasis.. Biochimie. 2004; 86: 839-848 [OpenAIRE] [PubMed]

Wälde, S, Kehlenbac, RH. The Part and the Whole: functions of nucleoporins in nucleocytoplasmic transport.. Trends Cell Biol. 2010; 20: 461-469 [OpenAIRE] [PubMed]

D'Angelo, MA, Hetzer, MW. Structure, dynamics and function of nuclear pore complexes.. Trends Cell Biol. 2008; 18: 456-466 [OpenAIRE] [PubMed]

Isgro, TA, Schulten, K. Cse1p-binding dynamics reveal a binding pattern for FG-repeat nucleoporins on transport receptors.. Structure. 2007; 15: 977-991 [OpenAIRE] [PubMed]

Isgro, TA, Schulten, K. Association of nuclear pore FG-repeat domains to NTF2 import and export complexes.. J Mol Biol. 2007; 366: 330-345 [PubMed]

Taylor, FR, Saucier, SE, Shown, EP, Parish, EJ, Kandutsch, AA. Correlation between oxysterol binding to a cytosolic binding protein and potency in the repression of hydroxymethylglutaryl coenzyme A reductase.. J Biol Chem. 1984; 259: 12382-12387 [PubMed]

Dawson, PA, Van der Westhuyzen, DR, Goldstein, JL, Brown, MS. Purification of oxysterol binding protein from hamster liver cytosol.. J Biol Chem. 1989; 264: 9046-9052 [PubMed]

Dawson, PA, Ridgway, ND, Slaughter, CA, Brown, MS, Goldstein, JL. cDNA cloning and expression of oxysterol-binding protein, an oligomer with a potential leucine zipper.. J Biol Chem. 1989; 264: 16798-16803 [OpenAIRE] [PubMed]

Perry, RJ, Ridgway, ND. Oxysterol-binding protein and vesicle-associated membrane protein-associated protein are required for sterol-dependent activation of the ceramide transport protein.. Mol Biol Cell. 2006; 17: 2604-2616 [OpenAIRE] [PubMed]

Wang, PY, Weng, J, Anderson, RG. OSBP is a cholesterol-regulated scaffolding protein in control of ERK 1/2 activation.. Science. 2005; 307: 1472-1476 [OpenAIRE] [PubMed]

Lehto, M, Olkkonen, VM. The OSBP-related proteins: a novel protein family involved in vesicle transport, cellular lipid metabolism, and cell signaling.. Biochim Biophys Acta. 2003; 1631: 1-11 [OpenAIRE] [PubMed]

Ridgway, ND. Oxysterol-binding proteins.. Subcell Biochem. 2010; 51: 159-182 [PubMed]

Raychaudhuri, S, Prinz, WA. The diverse functions of oxysterol-binding proteins.. Annu Rev Cell Dev Biol. 2010; 26: 157-177 [OpenAIRE] [PubMed]

51 references, page 1 of 4
Abstract
We earlier identified OSBP-related protein 8 (ORP8) as an endoplasmic reticulum oxysterol-binding protein implicated in cellular lipid homeostasis. We now investigated its action in hepatic cells in vivo and in vitro. Adenoviral overexpression of ORP8 in mouse liver induced a decrease of cholesterol, phospholipids, and triglycerides in serum (-34%, -26%, -37%, respectively) and liver tissue (-40%, -12%, -24%), coinciding with reduction of nuclear (n)SREBP-1 and -2 and mRNA levels of their target genes. Consistently, excess ORP8 reduced nSREBPs in HuH7 cells, and ORP8 overexpression or silencing by RNA interference moderately suppressed or induced the expression ...
Subjects
Medical Subject Headings: lipids (amino acids, peptides, and proteins)
free text keywords: Research Article, Biology, Model Organisms, Animal Models, Mouse, Molecular cell biology, Cellular Structures, Cell Nucleus, Gene expression, RNA interference, Membranes and Sorting, Medicine, R, Science, Q, 3111 Biomedicine, General Biochemistry, Genetics and Molecular Biology, General Agricultural and Biological Sciences, General Medicine, Molecular biology, Bimolecular fluorescence complementation, Hepatic stellate cell, Endoplasmic reticulum, Lipid metabolism, Sterol regulatory element-binding protein, Transfection, Nucleoporin
Related Organizations
Funded by
AKA| Cytoplasmic sterol sensors for integrated control of cellular lipid metabolism
Project
  • Funder: Academy of Finland (AKA)
  • Project Code: 121457
,
EC| LIPIDOMICNET
Project
LIPIDOMICNET
Lipid droplets as dynamic organelles of fat deposition and release: Translational research towards human disease
  • Funder: European Commission (EC)
  • Project Code: 202272
  • Funding stream: FP7 | SP1 | HEALTH
51 references, page 1 of 4

Horton, JD, Goldstein, JL, Brown, MS. SREBPs: activators of the complete program of cholesterol and fatty acid synthesis in the liver.. J Clin Invest. 2002; 109: 1125-1131 [OpenAIRE] [PubMed]

Goldstein, JL, DeBose-Boyd, RA, Brown, MS. Protein sensors for membrane sterols.. Cell. 2006; 124: 35-46 [OpenAIRE] [PubMed]

Eberle, D, Hegarty, B, Bossard, P, Ferre, P, Foufelle, F. SREBP transcription factors: master regulators of lipid homeostasis.. Biochimie. 2004; 86: 839-848 [OpenAIRE] [PubMed]

Wälde, S, Kehlenbac, RH. The Part and the Whole: functions of nucleoporins in nucleocytoplasmic transport.. Trends Cell Biol. 2010; 20: 461-469 [OpenAIRE] [PubMed]

D'Angelo, MA, Hetzer, MW. Structure, dynamics and function of nuclear pore complexes.. Trends Cell Biol. 2008; 18: 456-466 [OpenAIRE] [PubMed]

Isgro, TA, Schulten, K. Cse1p-binding dynamics reveal a binding pattern for FG-repeat nucleoporins on transport receptors.. Structure. 2007; 15: 977-991 [OpenAIRE] [PubMed]

Isgro, TA, Schulten, K. Association of nuclear pore FG-repeat domains to NTF2 import and export complexes.. J Mol Biol. 2007; 366: 330-345 [PubMed]

Taylor, FR, Saucier, SE, Shown, EP, Parish, EJ, Kandutsch, AA. Correlation between oxysterol binding to a cytosolic binding protein and potency in the repression of hydroxymethylglutaryl coenzyme A reductase.. J Biol Chem. 1984; 259: 12382-12387 [PubMed]

Dawson, PA, Van der Westhuyzen, DR, Goldstein, JL, Brown, MS. Purification of oxysterol binding protein from hamster liver cytosol.. J Biol Chem. 1989; 264: 9046-9052 [PubMed]

Dawson, PA, Ridgway, ND, Slaughter, CA, Brown, MS, Goldstein, JL. cDNA cloning and expression of oxysterol-binding protein, an oligomer with a potential leucine zipper.. J Biol Chem. 1989; 264: 16798-16803 [OpenAIRE] [PubMed]

Perry, RJ, Ridgway, ND. Oxysterol-binding protein and vesicle-associated membrane protein-associated protein are required for sterol-dependent activation of the ceramide transport protein.. Mol Biol Cell. 2006; 17: 2604-2616 [OpenAIRE] [PubMed]

Wang, PY, Weng, J, Anderson, RG. OSBP is a cholesterol-regulated scaffolding protein in control of ERK 1/2 activation.. Science. 2005; 307: 1472-1476 [OpenAIRE] [PubMed]

Lehto, M, Olkkonen, VM. The OSBP-related proteins: a novel protein family involved in vesicle transport, cellular lipid metabolism, and cell signaling.. Biochim Biophys Acta. 2003; 1631: 1-11 [OpenAIRE] [PubMed]

Ridgway, ND. Oxysterol-binding proteins.. Subcell Biochem. 2010; 51: 159-182 [PubMed]

Raychaudhuri, S, Prinz, WA. The diverse functions of oxysterol-binding proteins.. Annu Rev Cell Dev Biol. 2010; 26: 157-177 [OpenAIRE] [PubMed]

51 references, page 1 of 4
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