A comparison of two colorimetric assays, based upon Lowry and Bradford techniques, to estimate total protein in soil extracts

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Redmile-Gordon, M.A.; Armenise, E.; White, R.P.; Hirsch, P.R.; Goulding, K.W.T.;
  • Publisher: Pergamon Press
  • Journal: Soil Biology & Biochemistry,volume 67,issue 100,pages166-173 (issn: 0038-0717)
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  • Related identifiers: doi: 10.1016/j.soilbio.2013.08.017, pmc: PMC3819989
  • Subject: BRSP | Total protein determination | Microbiology | Humic acid | EPS | Extracellular polymeric substances | Soil Science | Article | Colorimetric protein assay | Glomalin related soil protein | GRSP | Soil microbial biofilm | Polyphenol interference

Soil extracts usually contain large quantities of dissolved humified organic material, typically reflected by high polyphenolic content. Since polyphenols seriously confound quantification of extracted protein, minimising this interference is important to ensure measure... View more
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