Development and validation of an antigen-binding capture ELISA for native and putrescine-modified anti-tetanus F(ab')2 fragments for the assessment of the cellular uptake and plasma kinetics of the antibodies.

Article English OPEN
Welfringer, Frédéric; D'Athis, Philippe; Scherrmann, Jean-Michel; Hervé, Françoise;
(2005)
  • Publisher: Elsevier
  • Related identifiers: doi: 10.1016/j.jim.2005.09.015
  • Subject: MESH: Animals | MESH: Male | MESH : HL-60 Cells | [SDV.IMM]Life Sciences [q-bio]/Immunology | MESH : Rats, Sprague-Dawley | MESH : Animals | MESH: Antibodies, Monoclonal | MESH: Endocytosis | MESH : Male | MESH: Putrescine | MESH: Enzyme-Linked Immunosorbent Assay | MESH : Antibodies, Monoclonal | MESH: Calibration | MESH: Isoelectric Focusing | MESH : Putrescine | MESH : Immunoglobulin Fab Fragments | MESH : Endocytosis | MESH: Tetanus Toxoid | MESH : Toxoids | MESH: Immunoglobulin Fab Fragments | MESH : Isoelectric Focusing | MESH: HL-60 Cells | MESH: Rats | MESH : Cations | MESH : Tetanus Toxoid | MESH: Humans | MESH: Rats, Sprague-Dawley | MESH : Rats | MESH: Reproducibility of Results | MESH : Calibration | MESH: Cations | MESH : Enzyme-Linked Immunosorbent Assay | MESH : Reproducibility of Results | MESH: Toxoids | MESH: Horses | MESH : Humans | MESH : Horses | MESH: Antibody Specificity | MESH : Antibody Specificity | [ SDV.IMM ] Life Sciences [q-bio]/Immunology

International audience; Cationization is a strategy to enhance the permeability of antibodies to physiological membranes for potential therapeutic and diagnostic applications of these proteins, with one of its crucial points being the retention of antigen binding activi... View more
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