Lipopolyamine-mediated transfection of reporter plasmids into a fish cell line

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Villalobos, Patricio; Rojas, M. Verónica; Conejeros, Pablo; Marshall, Sergio H.;

Conditions have been optimised to transfect the fish cell line CHSE-214 to measure expression, maintenance and putative chromosomal integration of the reporter gene LUC, spliced into two versions of an expression vector. The first is pCMVL, and the second p103, a novel ... View more