publication . Article . 2009

confocal detection of planar homogeneous and heterogeneous immunosorbent assays

Ghafari, Homanaz; Zhou, Yanzhou; Ali, Selman; Hanley, Quentin S.;
Open Access
  • Published: 01 Jan 2009 Journal: Journal of Biomedical Optics, volume 14, page 64,022 (issn: 1083-3668, Copyright policy)
  • Publisher: SPIE-Intl Soc Optical Eng
Optically sectioned detection of fluorescence immunoassays using a confocal microscope enables the creation of both homo- and heterogeneous planar format assays. We report a set assays requiring optically sectioned detection using a model system and analysis procedures for separating signals of a surface layer from an overlying solution. A model sandwich assay with human immunoglobulin G as the target antigen is created on a glass substrate. The prepared surfaces are exposed to antigen and a FITC-labeled secondary antibody. The resulting preparations are either read directly to provide a homogeneous assay or after wash steps, giving a heterogeneous assay. The si...
free text keywords: Atomic and Molecular Physics, and Optics, Electronic, Optical and Magnetic Materials, Biomaterials, Biomedical Engineering, Overlayer, Fluorescence, Confocal, Detection limit, Planar, Analyte, Luminescence, Medicine, business.industry, business, Optics, Microscope, law.invention, law
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