A chemical library to screen protein and protein-ligand crystallization using a versatile microfluidic platform

Article, Preprint English OPEN
Gerard , Charline ,; Ferry , Gilles; Vuillard , Laurent ,; Boutin , Jean ,; Ferte , Nathalie ,; Grossier , Romain ,; Candoni , Nadine ,; Veesler , Stéphane ,;
  • Publisher: HAL CCSD
  • Related identifiers: doi: 10.1021/acs.cgd.8b00572
  • Subject: Co-crystallization | [ SDV.BBM.BS ] Life Sciences [q-bio]/Biochemistry, Molecular Biology/Biomolecules [q-bio.BM] | [ SPI.GPROC ] Engineering Sciences [physics]/Chemical and Process Engineering | [SPI.GPROC]Engineering Sciences [physics]/Chemical and Process Engineering | Crystallization | [PHYS.COND.CM-MS]Physics [physics]/Condensed Matter [cond-mat]/Materials Science [cond-mat.mtrl-sci] | Microfluidic | Biological macromolecules | [ PHYS.COND.CM-MS ] Physics [physics]/Condensed Matter [cond-mat]/Materials Science [cond-mat.mtrl-sci] | [SDV.BBM.BS]Life Sciences [q-bio]/Biochemistry, Molecular Biology/Biomolecules [q-bio.BM]

International audience; Here, we describe a plug-and-play microfluidic platform, suitable for protein crystallization. The droplet factory is designed to generate hundreds of droplets as small as a few nanoliters (2 to 10nL) for screening and optimization of crystalliza... View more
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