EZ spheres: A stable and expandable culture system for the generation of pre-rosette multipotent stem cells from human ESCs and iPSCs

Article, Other literature type English OPEN
Ebert, Allison D.; Shelley, Brandon C.; Hurley, Amanda M.; Onorati, Marco; Castiglioni, Valentina; Patitucci, Teresa N.; Svendsen, Soshana P.; Mattis, Virginia B.; McGivern, Jered V.; Schwab, Andrew J.; Sareen, Dhruv; Kim, Ho Won; Cattaneo, Elena; Svendsen, Clive N.;

We have developed a simple method to generate and expand multipotent, self-renewing pre-rosette neural stem cells from both human embryonic stem cells (hESCs) and human induced pluripotent stem cells (iPSCs) without utilizing embryoid body formation, manual selection te... View more
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