publication . Article . Other literature type . 2019

Affimer reagents as tools in diagnosing plant virus diseases

Hesketh, E; Tiede, C; Adamson, H; Adams, T; Byrne, M; Meshcheriakova, Y; Kruse, I; McPherson, M; Lomonossoff, G; Tomlinson, D; ...
Open Access English
  • Published: 17 May 2019
  • Publisher: Nature Publishing Group
  • Country: United Kingdom
Abstract
Abstract Plant viruses can cause devastating losses to agriculture and are therefore a major threat to food security. The rapid identification of virally-infected crops allowing containment is essential to limit such threats, but plant viral diseases can be extremely challenging to diagnose. An ideal method for plant virus diagnosis would be a device which can be implemented easily in the field. Such devices require a binding reagent that is specific for the virus of interest. We chose to investigate the use of Affimer reagents, artificial binding proteins and a model plant virus Cowpea Mosaic virus (CPMV) empty virus like particles (eVLPs). CPMV-eVLP mimic the ...
Subjects
Medical Subject Headings: food and beveragesvirusesfungi
free text keywords: Multidisciplinary, Medicine, R, Science, Q, Article, Assay systems, Cryoelectron microscopy
Related Organizations
Funded by
RCUK| Untangling the processes of replication in and encapsidation in Picornavirales
Project
  • Funder: Research Council UK (RCUK)
  • Project Code: BB/L021250/1
  • Funding stream: BBSRC
,
RCUK| Untangling the processes of replication and encapsidation in Picornavirales
Project
  • Funder: Research Council UK (RCUK)
  • Project Code: BB/L020955/1
  • Funding stream: BBSRC
,
WT| New infrastructure for EM studies at the University of Leeds.
Project
  • Funder: Wellcome Trust (WT)
  • Project Code: 090932
  • Funding stream: Cellular and Molecular Neuroscience
,
RCUK| Enhanced Research Capacity
Project
  • Funder: Research Council UK (RCUK)
  • Project Code: BBS/E/J/000PR9794
  • Funding stream: BBSRC
,
RCUK| Exploiting the power of heterologous expression in plants to discover new virus structure.
Project
  • Funder: Research Council UK (RCUK)
  • Project Code: BB/R00160X/1
  • Funding stream: BBSRC
35 references, page 1 of 3

Donoso, A, Valenzuela, S. In-field molecular diagnosis of plant pathogens: recent trends and future perspectives. Plant Pathology.. 2018; 67 (7): 1451-1461 [OpenAIRE] [DOI]

Khater, M, la Escosura-Muniz de, A, Merkoci, A. Biosensors for plant pathogen detection. Biosens Bioelectron.. 2017; 93: 72-86 [OpenAIRE] [PubMed] [DOI]

Tiede, C. Adhiron: a stable and versatile peptide display scaffold for molecular recognition applications. Protein Engineering, Design and Selection.. 2014; 27 (5): 145-155 [OpenAIRE] [DOI]

Tiede, C. Affimer proteins are versatile and renewable affinity reagents. eLife.. 2017; 6: e24903 [OpenAIRE] [PubMed] [DOI]

Tang, AA-S, Tiede, C, Hughes, DJ, McPherson, MJ, Tomlinson, DC. Isolation of isoform-specific binding proteins (Affimers) by phage display using negative selection. Sci Signal.. 2017; 10 (505): eaan0868 [OpenAIRE] [PubMed] [DOI]

Zhurauski, P. Sensitive and selective Affimer-functionalised interdigitated electrode-based capacitive biosensor for Her4 protein tumour biomarker detection. Biosens Bioelectron.. 2018; 108: 1-8 [OpenAIRE] [PubMed] [DOI]

Xie, C. Development of an Affimer-antibody combined immunological diagnosis kit for glypican-3. Scientific Reports. 2017; 7 (1): 9608 [OpenAIRE] [PubMed] [DOI]

Robinson, JI. Affimer proteins inhibit immune complex binding to FcγRIIIa with high specificity through competitive and allosteric modes of action. Proc Natl Acad Sci USA. 2018; 115 (1): E72-E81 [OpenAIRE] [PubMed] [DOI]

Lafforgue, G, Sardanyes, J, Elena, SF. Differences in accumulation and virulence determine the outcome of competition during Tobacco etch virus coinfection. PLoS One.. 2011; 6 (3): e17917 [OpenAIRE] [PubMed] [DOI]

Steele, JFC. Synthetic plant virology for nanobiotechnology and nanomedicine. Wiley Interdiscip Rev Nanomed Nanobiotechnol.. 2017; 9 (4): e1447 [OpenAIRE] [DOI]

Meshcheriakova, Y, Durrant, A, Hesketh, EL, Ranson, NA, Lomonossoff, GP. Combining high-resolution cryo-electron microscopy and mutagenesis to develop cowpea mosaic virus for bionanotechnology. Biochm Soc Trans.. 2017; 45 (6): 1263-1269 [OpenAIRE] [DOI]

Saunders, K, Sainsbury, F, Lomonossoff, GP. Efficient generation of cowpea mosaic virus empty virus-like particles by the proteolytic processing of precursors in insect cells and plants. Virology.. 2009; 393 (2): 329-337 [OpenAIRE] [PubMed] [DOI]

Hesketh, EL. Mechanisms of assembly and genome packaging in an RNA virus revealed by high-resolution cryo-EM. Nature. Communications.. 2015; 6: 10113 [OpenAIRE]

Hesketh, EL, Meshcheriakova, Y, Thompson, RF, Lomonossoff, GP, Ranson, NA. The structures of a naturally empty cowpea mosaic virus particle and its genome-containing counterpart by cryo-electron microscopy. Scientific Reports. 2017; 7 (1): 539 [OpenAIRE] [PubMed] [DOI]

Huynh, NT. Crystal Structure and Proteomics Analysis of Empty Virus-like Particles of Cowpea Mosaic Virus. Structure.. 2016; 24 (4): 567-575 [OpenAIRE] [PubMed] [DOI]

35 references, page 1 of 3
Abstract
Abstract Plant viruses can cause devastating losses to agriculture and are therefore a major threat to food security. The rapid identification of virally-infected crops allowing containment is essential to limit such threats, but plant viral diseases can be extremely challenging to diagnose. An ideal method for plant virus diagnosis would be a device which can be implemented easily in the field. Such devices require a binding reagent that is specific for the virus of interest. We chose to investigate the use of Affimer reagents, artificial binding proteins and a model plant virus Cowpea Mosaic virus (CPMV) empty virus like particles (eVLPs). CPMV-eVLP mimic the ...
Subjects
Medical Subject Headings: food and beveragesvirusesfungi
free text keywords: Multidisciplinary, Medicine, R, Science, Q, Article, Assay systems, Cryoelectron microscopy
Related Organizations
Funded by
RCUK| Untangling the processes of replication in and encapsidation in Picornavirales
Project
  • Funder: Research Council UK (RCUK)
  • Project Code: BB/L021250/1
  • Funding stream: BBSRC
,
RCUK| Untangling the processes of replication and encapsidation in Picornavirales
Project
  • Funder: Research Council UK (RCUK)
  • Project Code: BB/L020955/1
  • Funding stream: BBSRC
,
WT| New infrastructure for EM studies at the University of Leeds.
Project
  • Funder: Wellcome Trust (WT)
  • Project Code: 090932
  • Funding stream: Cellular and Molecular Neuroscience
,
RCUK| Enhanced Research Capacity
Project
  • Funder: Research Council UK (RCUK)
  • Project Code: BBS/E/J/000PR9794
  • Funding stream: BBSRC
,
RCUK| Exploiting the power of heterologous expression in plants to discover new virus structure.
Project
  • Funder: Research Council UK (RCUK)
  • Project Code: BB/R00160X/1
  • Funding stream: BBSRC
35 references, page 1 of 3

Donoso, A, Valenzuela, S. In-field molecular diagnosis of plant pathogens: recent trends and future perspectives. Plant Pathology.. 2018; 67 (7): 1451-1461 [OpenAIRE] [DOI]

Khater, M, la Escosura-Muniz de, A, Merkoci, A. Biosensors for plant pathogen detection. Biosens Bioelectron.. 2017; 93: 72-86 [OpenAIRE] [PubMed] [DOI]

Tiede, C. Adhiron: a stable and versatile peptide display scaffold for molecular recognition applications. Protein Engineering, Design and Selection.. 2014; 27 (5): 145-155 [OpenAIRE] [DOI]

Tiede, C. Affimer proteins are versatile and renewable affinity reagents. eLife.. 2017; 6: e24903 [OpenAIRE] [PubMed] [DOI]

Tang, AA-S, Tiede, C, Hughes, DJ, McPherson, MJ, Tomlinson, DC. Isolation of isoform-specific binding proteins (Affimers) by phage display using negative selection. Sci Signal.. 2017; 10 (505): eaan0868 [OpenAIRE] [PubMed] [DOI]

Zhurauski, P. Sensitive and selective Affimer-functionalised interdigitated electrode-based capacitive biosensor for Her4 protein tumour biomarker detection. Biosens Bioelectron.. 2018; 108: 1-8 [OpenAIRE] [PubMed] [DOI]

Xie, C. Development of an Affimer-antibody combined immunological diagnosis kit for glypican-3. Scientific Reports. 2017; 7 (1): 9608 [OpenAIRE] [PubMed] [DOI]

Robinson, JI. Affimer proteins inhibit immune complex binding to FcγRIIIa with high specificity through competitive and allosteric modes of action. Proc Natl Acad Sci USA. 2018; 115 (1): E72-E81 [OpenAIRE] [PubMed] [DOI]

Lafforgue, G, Sardanyes, J, Elena, SF. Differences in accumulation and virulence determine the outcome of competition during Tobacco etch virus coinfection. PLoS One.. 2011; 6 (3): e17917 [OpenAIRE] [PubMed] [DOI]

Steele, JFC. Synthetic plant virology for nanobiotechnology and nanomedicine. Wiley Interdiscip Rev Nanomed Nanobiotechnol.. 2017; 9 (4): e1447 [OpenAIRE] [DOI]

Meshcheriakova, Y, Durrant, A, Hesketh, EL, Ranson, NA, Lomonossoff, GP. Combining high-resolution cryo-electron microscopy and mutagenesis to develop cowpea mosaic virus for bionanotechnology. Biochm Soc Trans.. 2017; 45 (6): 1263-1269 [OpenAIRE] [DOI]

Saunders, K, Sainsbury, F, Lomonossoff, GP. Efficient generation of cowpea mosaic virus empty virus-like particles by the proteolytic processing of precursors in insect cells and plants. Virology.. 2009; 393 (2): 329-337 [OpenAIRE] [PubMed] [DOI]

Hesketh, EL. Mechanisms of assembly and genome packaging in an RNA virus revealed by high-resolution cryo-EM. Nature. Communications.. 2015; 6: 10113 [OpenAIRE]

Hesketh, EL, Meshcheriakova, Y, Thompson, RF, Lomonossoff, GP, Ranson, NA. The structures of a naturally empty cowpea mosaic virus particle and its genome-containing counterpart by cryo-electron microscopy. Scientific Reports. 2017; 7 (1): 539 [OpenAIRE] [PubMed] [DOI]

Huynh, NT. Crystal Structure and Proteomics Analysis of Empty Virus-like Particles of Cowpea Mosaic Virus. Structure.. 2016; 24 (4): 567-575 [OpenAIRE] [PubMed] [DOI]

35 references, page 1 of 3
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