publication . Article . 2014

Highly efficient differentiation of embryonic stem cells into adipocytes by ascorbic acid

Cuaranta-Monroy, Ixchelt; Simandi, Zoltan; Kolostyak, Zsuzsanna; Doan-Xuan, Quang-Minh; Poliska, Szilard; Horvath, Attila; Nagy, Gergely; Bacso, Zsolt; Nagy, Laszlo;
Open Access English
  • Published: 01 Jul 2014 Journal: volume 13, issue 1, pages 88-97issn: 1873-5061, eissn: 1876-7753, Copyright policy
  • Publisher: Elsevier BV
Abstract
Adipocyte differentiation and function have become the major research targets due to the increasing interest in obesity and related metabolic conditions. Although, late stages of adipogenesis have been extensively studied, the early phases remain poorly understood. Here we present that supplementing ascorbic acid (AsA) to the adipogenic differentiation cocktail enables the robust and efficient differentiation of mouse embryonic stem cells (mESCs) to mature adipocytes. Such ESC-derived adipocytes mimic the gene-expression profile of subcutaneous isolated adipocytes in vivo remarkably well, much closer than 3T3-L1 derived ones. Moreover, the differentiated cells a...
Subjects
free text keywords: Orvostudományok, Cell Biology, Developmental Biology, Klinikai orvostudományok, Medicine(all), Biology (General), QH301-705.5
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publication . Article . 2014

Highly efficient differentiation of embryonic stem cells into adipocytes by ascorbic acid

Cuaranta-Monroy, Ixchelt; Simandi, Zoltan; Kolostyak, Zsuzsanna; Doan-Xuan, Quang-Minh; Poliska, Szilard; Horvath, Attila; Nagy, Gergely; Bacso, Zsolt; Nagy, Laszlo;