publication . Article . Other literature type . 2018

Minimal information for studies of extracellular vesicles 2018 (MISEV2018) : a position statement of the International Society for Extracellular Vesicles and update of the MISEV2014 guidelines

Théry, Clotilde; Witwer, Kenneth W; Aikawa, Elena; Alcaraz, Maria Jose; Anderson, Johnathon D; Andriantsitohaina, Ramaroson; Antoniou, Anna; Arab, Tanina; Archer, Fabienne; Atkin-Smith, Georgia K; ...
Open Access English
  • Published: 23 Nov 2018
  • Publisher: HAL CCSD
Abstract
Ministry of Education, and grants from INCa (INCA-11548), French National Research Agency (ANR-10-IDEX-0001-02 PSL* and ANR-11-LABX-0043), SIDACTION (17-1-AAE-1138), Fondation ARC (PGA1 RF20180206962, PJA 20171206453). KWW and CT receive support from NIDA (DA040385)
Subjects
free text keywords: ectosomes, exosomes, extracellular vesicles, guidelines, microparticles, microvesicles, minimal information requirements, reproducibility, rigor, standardization, [SDV]Life Sciences [q-bio], extracellular vesicles; exosomes; ectosomes; microvesicles; minimal information requirements; guidelines; standardization; microparticles; rigor; reproducibility, Science & Technology, Life Sciences & Biomedicine, Cell Biology, CELL-DERIVED MICROPARTICLES, SURFACE-PLASMON RESONANCE, SIZE-EXCLUSION CHROMATOGRAPHY, FIELD-FLOW FRACTIONATION, HUMAN URINARY EXOSOMES, PROSTATE-CANCER, MEMBRANE-VESICLES, FETAL BOVINE, CIRCULATING MICROPARTICLES, PREANALYTICAL PARAMETERS, ectosomes; exosomes; extracellular vesicles; guidelines; microparticles; microvesicles; minimal information requirements; reproducibility; rigor; standardization; Histology; Cell Biology, Vesículas extracelulares, Exossomos, Micropartículas derivadas de células, /dk/atira/pure/researchoutput/pubmedpublicationtype/D016428, Journal Article, [SDV.BC]Life Sciences [q-bio]/Cellular Biology, extracellular vesicles ; exosomes ; ectosomes ; microvesicles ; minimalinformation requirements ; guidelines ; standardization ; microparticles ; rigor ; reproducibility, Biology, Human medicine, requirements, Position Paper, extracellular vesicles;exosomes;ectosomes;microvesicles;minimal information requirements;guidelines;standardization;microparticles;rigor;reproducibility, [SPI.ACOU]Engineering Sciences [physics]/Acoustics [physics.class-ph], [SPI.MAT]Engineering Sciences [physics]/Materials, [SPI.NANO]Engineering Sciences [physics]/Micro and nanotechnologies/Microelectronics, ectosomes; exosomes; extracellular vesicles; guidelines; microparticles; microvesicles; minimal information requirements; reproducibility; rigor; standardization, Biochemistry and Cell Biology, size-exclusion, chromatography, circulating, Science & Technology, Life Sciences & Biomedicine, Cell Biology, extracellular vesicles, exosomes, ectosomes, microvesicles, minimal information requirements, guidelines, standardization, microparticles, rigor, reproducibility, CELL-DERIVED MICROPARTICLES, SURFACE-PLASMON RESONANCE, SIZE-EXCLUSION CHROMATOGRAPHY, FIELD-FLOW FRACTIONATION, HUMAN URINARY EXOSOMES, PROSTATE-CANCER, MEMBRANE-VESICLES, FETAL BOVINE, CIRCULATING MICROPARTICLES, PREANALYTICAL PARAMETERS, Interacció cel·lular, Membranes cel·lulars, Cell interaction, Cell membranes, ectosomes ; exosomes ; extracellular vesicles ; guidelines ; microparticles ; microvesicles ; minimal information requirements ; reproducibility ; rigor ; standardization, 1182 Biochemistry, cell and molecular biology, Medicine and Health Sciences, 570, Histology, Cytology, QH573-671, ddc:610, ddc:570, Chemistry, Position statement
Funded by
NIH| Discovering novel players in mechanisms of extracellular vesicle release, cargo loading, and early pathogenesis of late-onset Alzheimer's Disease
Project
  • Funder: National Institutes of Health (NIH)
  • Project Code: 1R56AG057430-01
  • Funding stream: NATIONAL INSTITUTE ON AGING
,
NIH| Extracellular Vesicle and Extracellular RNA Biomarkers of HIV-1 Central Nervous System Pathogenesis and Cigarette Use
Project
  • Funder: National Institutes of Health (NIH)
  • Project Code: 1R01DA047807-01
  • Funding stream: NATIONAL INSTITUTE ON DRUG ABUSE
,
EC| PARAGEN
Project
PARAGEN
Biomaterials with incorporated MSC-secreted PARAcrine molecules for bone reGENeration
  • Funder: European Commission (EC)
  • Project Code: 708711
  • Funding stream: H2020 | MSCA-IF-GF
,
ANR| DCBIOL
Project
DCBIOL
Biologie des cellules dendritiques
  • Funder: French National Research Agency (ANR) (ANR)
  • Project Code: 11-LABX-0043
,
EC| evFOUNDRY
Project
evFOUNDRY
The Extracellular Vesicle Foundry
  • Funder: European Commission (EC)
  • Project Code: 801367
  • Funding stream: H2020 | RIA
Communities
FET H2020FET OPEN: FET-Open research and innovation actions
FET H2020FET OPEN: The Extracellular Vesicle Foundry
FET H2020FET OPEN: FET-Open research and innovation actions
FET H2020FET OPEN: Extracellular vesicles from a natural source for tailor-made nanomaterials
327 references, page 1 of 22

Clotilde Théry http://orcid.org/0000-0001-8294-6884 Kenneth W Witwer http://orcid.org/0000-0003-1664-4233 References, especially those provided to illustrate methods and approaches, are representative only, and are not meant to be a comprehensive review of the literature. Most references were derived from suggestions provided in the MISEV2018 Survey results. Each reference was checked by multiple authors. Citation implies deemed relevance of scientific content and not an endorsement by the authors or ISEV of any particular journal or editorial practice.

[1] Lotvall J, Hill AF, Hochberg F, et al. Minimal experimental requirements for definition of extracellular vesicles and their functions: a position statement from the international society for extracellular vesicles. J Extracell Vesicles. 2014;3:26913. Available from: http://www.ncbi.nlm.nih.gov/pubmed/25536934 [2] Witwer KW, Soekmadji C, Hill AF, et al. Updating the MISEV minimal requirements for extracellular vesicle studies: building bridges to reproducibility. J Extracell Vesicles. 2017;6(1):1396823. Available from: https:// www.tandfonline.com/doi/full/10.1080/20013078.2017. [OpenAIRE]

1396823 [3] Stein JM, Luzio JP Ectocytosis caused by sublytic autologous complement attack on human neutrophils. The sorting of endogenous plasma-membrane proteins and lipids into shed vesicles. Biochem J. 1991;274 (Pt 2):381-386. Available from: http://www.ncbi.nlm.

nih.gov/pubmed/1848755 [4] Cocucci E, Meldolesi J Ectosomes and exosomes: shedding the confusion between extracellular vesicles.

Trends Cell Biol. 2015;25(6):364-372. Available from: http://www.ncbi.nlm.nih.gov/pubmed/25683921 [5] Gould SJ, Raposo G As we wait: coping with an imperfect nomenclature for extracellular vesicles.

J Extracell Vesicles. 2013;2. Available from: http:// www.ncbi.nlm.nih.gov/pubmed/24009890 [6] Gardiner C, Di Vizio D, Sahoo S, et al. Techniques used for the isolation and characterization of extracellular vesicles: results of a worldwide survey. J Extracell Vesicles. 2016;5:32945. Available from: http://www.

ncbi.nlm.nih.gov/pubmed/27802845 [7] Rojas A The imperative authentication of cell lines.

Antimicrob Agents Chemother. 2017;61(11):e01823- 17. Available from: http://aac.asm.org/lookup/doi/10.

1128/AAC.01823-17 [8] Reid Y, Storts D, Riss T, et al. Authentication of human cell lines by STR DNA profiling analysis [Internet]. Assay Guidance Manual. 2004. Available from: http://www.ncbi.nlm.nih.gov/pubmed/23805434 [9] Chen TS, Arslan F, Yin Y, et al. Enabling a robust scalable manufacturing process for therapeutic exosomes through oncogenic immortalization of human ESC-derived MSCs. J Transl Med. 2011;9(1):47.

Available from: http://translational-medicine.biomed central.com/articles/10.1186/1479-5876-9-47 [10] Lima LG, Chammas R, Monteiro RQ, et al. Tumorderived microvesicles modulate the establishment of metastatic melanoma in a phosphatidylserine-dependent manner. Cancer Lett. 2009;283(2):168-175. Available from: http://linkinghub.elsevier.com/retrieve/pii/ S0304383509002420 [11] Frey B, Gaipl US The immune functions of phosphatidylserine in membranes of dying cells and microvesicles.

Semin Immunopathol. 2011;33(5):497-516. Available from: http://link.springer.com/10.1007/s00281-010- 0228-6 [12] Roseblade A, Luk F, Ung A, et al. Targeting microparticle biogenesis: a novel approach to the circumvention of cancer multidrug resistance. Curr Cancer Drug Targets. 2015;15(3):205-214. Available from: http:// www.ncbi.nlm.nih.gov/pubmed/25714701 [13] Takasugi M Emerging roles of extracellular vesicles in cellular senescence and aging. Aging Cell. 2018;17(2): e12734.

[14] Patel DB, Gray KM, Santharam Y, et al. Impact of cell culture parameters on production and vascularization bioactivity of mesenchymal stem cell-derived extracellular vesicles. Bioeng Transl Med. 2017;2(2):170-179. [OpenAIRE]

[15] Dang VD, Jella KK, Ragheb RRT, et al. Lipidomic and proteomic analysis of exosomes from mouse cortical collecting duct cells. FASEB J. 2017;31(12):5399-5408.

Available from: http://www.fasebj.org/doi/10.1096/fj.

201700417R [16] Klingeborn M, Dismuke WM, Skiba NP, et al.

327 references, page 1 of 22
Abstract
Ministry of Education, and grants from INCa (INCA-11548), French National Research Agency (ANR-10-IDEX-0001-02 PSL* and ANR-11-LABX-0043), SIDACTION (17-1-AAE-1138), Fondation ARC (PGA1 RF20180206962, PJA 20171206453). KWW and CT receive support from NIDA (DA040385)
Subjects
free text keywords: ectosomes, exosomes, extracellular vesicles, guidelines, microparticles, microvesicles, minimal information requirements, reproducibility, rigor, standardization, [SDV]Life Sciences [q-bio], extracellular vesicles; exosomes; ectosomes; microvesicles; minimal information requirements; guidelines; standardization; microparticles; rigor; reproducibility, Science & Technology, Life Sciences & Biomedicine, Cell Biology, CELL-DERIVED MICROPARTICLES, SURFACE-PLASMON RESONANCE, SIZE-EXCLUSION CHROMATOGRAPHY, FIELD-FLOW FRACTIONATION, HUMAN URINARY EXOSOMES, PROSTATE-CANCER, MEMBRANE-VESICLES, FETAL BOVINE, CIRCULATING MICROPARTICLES, PREANALYTICAL PARAMETERS, ectosomes; exosomes; extracellular vesicles; guidelines; microparticles; microvesicles; minimal information requirements; reproducibility; rigor; standardization; Histology; Cell Biology, Vesículas extracelulares, Exossomos, Micropartículas derivadas de células, /dk/atira/pure/researchoutput/pubmedpublicationtype/D016428, Journal Article, [SDV.BC]Life Sciences [q-bio]/Cellular Biology, extracellular vesicles ; exosomes ; ectosomes ; microvesicles ; minimalinformation requirements ; guidelines ; standardization ; microparticles ; rigor ; reproducibility, Biology, Human medicine, requirements, Position Paper, extracellular vesicles;exosomes;ectosomes;microvesicles;minimal information requirements;guidelines;standardization;microparticles;rigor;reproducibility, [SPI.ACOU]Engineering Sciences [physics]/Acoustics [physics.class-ph], [SPI.MAT]Engineering Sciences [physics]/Materials, [SPI.NANO]Engineering Sciences [physics]/Micro and nanotechnologies/Microelectronics, ectosomes; exosomes; extracellular vesicles; guidelines; microparticles; microvesicles; minimal information requirements; reproducibility; rigor; standardization, Biochemistry and Cell Biology, size-exclusion, chromatography, circulating, Science & Technology, Life Sciences & Biomedicine, Cell Biology, extracellular vesicles, exosomes, ectosomes, microvesicles, minimal information requirements, guidelines, standardization, microparticles, rigor, reproducibility, CELL-DERIVED MICROPARTICLES, SURFACE-PLASMON RESONANCE, SIZE-EXCLUSION CHROMATOGRAPHY, FIELD-FLOW FRACTIONATION, HUMAN URINARY EXOSOMES, PROSTATE-CANCER, MEMBRANE-VESICLES, FETAL BOVINE, CIRCULATING MICROPARTICLES, PREANALYTICAL PARAMETERS, Interacció cel·lular, Membranes cel·lulars, Cell interaction, Cell membranes, ectosomes ; exosomes ; extracellular vesicles ; guidelines ; microparticles ; microvesicles ; minimal information requirements ; reproducibility ; rigor ; standardization, 1182 Biochemistry, cell and molecular biology, Medicine and Health Sciences, 570, Histology, Cytology, QH573-671, ddc:610, ddc:570, Chemistry, Position statement
Funded by
NIH| Discovering novel players in mechanisms of extracellular vesicle release, cargo loading, and early pathogenesis of late-onset Alzheimer's Disease
Project
  • Funder: National Institutes of Health (NIH)
  • Project Code: 1R56AG057430-01
  • Funding stream: NATIONAL INSTITUTE ON AGING
,
NIH| Extracellular Vesicle and Extracellular RNA Biomarkers of HIV-1 Central Nervous System Pathogenesis and Cigarette Use
Project
  • Funder: National Institutes of Health (NIH)
  • Project Code: 1R01DA047807-01
  • Funding stream: NATIONAL INSTITUTE ON DRUG ABUSE
,
EC| PARAGEN
Project
PARAGEN
Biomaterials with incorporated MSC-secreted PARAcrine molecules for bone reGENeration
  • Funder: European Commission (EC)
  • Project Code: 708711
  • Funding stream: H2020 | MSCA-IF-GF
,
ANR| DCBIOL
Project
DCBIOL
Biologie des cellules dendritiques
  • Funder: French National Research Agency (ANR) (ANR)
  • Project Code: 11-LABX-0043
,
EC| evFOUNDRY
Project
evFOUNDRY
The Extracellular Vesicle Foundry
  • Funder: European Commission (EC)
  • Project Code: 801367
  • Funding stream: H2020 | RIA
Communities
FET H2020FET OPEN: FET-Open research and innovation actions
FET H2020FET OPEN: The Extracellular Vesicle Foundry
FET H2020FET OPEN: FET-Open research and innovation actions
FET H2020FET OPEN: Extracellular vesicles from a natural source for tailor-made nanomaterials
327 references, page 1 of 22

Clotilde Théry http://orcid.org/0000-0001-8294-6884 Kenneth W Witwer http://orcid.org/0000-0003-1664-4233 References, especially those provided to illustrate methods and approaches, are representative only, and are not meant to be a comprehensive review of the literature. Most references were derived from suggestions provided in the MISEV2018 Survey results. Each reference was checked by multiple authors. Citation implies deemed relevance of scientific content and not an endorsement by the authors or ISEV of any particular journal or editorial practice.

[1] Lotvall J, Hill AF, Hochberg F, et al. Minimal experimental requirements for definition of extracellular vesicles and their functions: a position statement from the international society for extracellular vesicles. J Extracell Vesicles. 2014;3:26913. Available from: http://www.ncbi.nlm.nih.gov/pubmed/25536934 [2] Witwer KW, Soekmadji C, Hill AF, et al. Updating the MISEV minimal requirements for extracellular vesicle studies: building bridges to reproducibility. J Extracell Vesicles. 2017;6(1):1396823. Available from: https:// www.tandfonline.com/doi/full/10.1080/20013078.2017. [OpenAIRE]

1396823 [3] Stein JM, Luzio JP Ectocytosis caused by sublytic autologous complement attack on human neutrophils. The sorting of endogenous plasma-membrane proteins and lipids into shed vesicles. Biochem J. 1991;274 (Pt 2):381-386. Available from: http://www.ncbi.nlm.

nih.gov/pubmed/1848755 [4] Cocucci E, Meldolesi J Ectosomes and exosomes: shedding the confusion between extracellular vesicles.

Trends Cell Biol. 2015;25(6):364-372. Available from: http://www.ncbi.nlm.nih.gov/pubmed/25683921 [5] Gould SJ, Raposo G As we wait: coping with an imperfect nomenclature for extracellular vesicles.

J Extracell Vesicles. 2013;2. Available from: http:// www.ncbi.nlm.nih.gov/pubmed/24009890 [6] Gardiner C, Di Vizio D, Sahoo S, et al. Techniques used for the isolation and characterization of extracellular vesicles: results of a worldwide survey. J Extracell Vesicles. 2016;5:32945. Available from: http://www.

ncbi.nlm.nih.gov/pubmed/27802845 [7] Rojas A The imperative authentication of cell lines.

Antimicrob Agents Chemother. 2017;61(11):e01823- 17. Available from: http://aac.asm.org/lookup/doi/10.

1128/AAC.01823-17 [8] Reid Y, Storts D, Riss T, et al. Authentication of human cell lines by STR DNA profiling analysis [Internet]. Assay Guidance Manual. 2004. Available from: http://www.ncbi.nlm.nih.gov/pubmed/23805434 [9] Chen TS, Arslan F, Yin Y, et al. Enabling a robust scalable manufacturing process for therapeutic exosomes through oncogenic immortalization of human ESC-derived MSCs. J Transl Med. 2011;9(1):47.

Available from: http://translational-medicine.biomed central.com/articles/10.1186/1479-5876-9-47 [10] Lima LG, Chammas R, Monteiro RQ, et al. Tumorderived microvesicles modulate the establishment of metastatic melanoma in a phosphatidylserine-dependent manner. Cancer Lett. 2009;283(2):168-175. Available from: http://linkinghub.elsevier.com/retrieve/pii/ S0304383509002420 [11] Frey B, Gaipl US The immune functions of phosphatidylserine in membranes of dying cells and microvesicles.

Semin Immunopathol. 2011;33(5):497-516. Available from: http://link.springer.com/10.1007/s00281-010- 0228-6 [12] Roseblade A, Luk F, Ung A, et al. Targeting microparticle biogenesis: a novel approach to the circumvention of cancer multidrug resistance. Curr Cancer Drug Targets. 2015;15(3):205-214. Available from: http:// www.ncbi.nlm.nih.gov/pubmed/25714701 [13] Takasugi M Emerging roles of extracellular vesicles in cellular senescence and aging. Aging Cell. 2018;17(2): e12734.

[14] Patel DB, Gray KM, Santharam Y, et al. Impact of cell culture parameters on production and vascularization bioactivity of mesenchymal stem cell-derived extracellular vesicles. Bioeng Transl Med. 2017;2(2):170-179. [OpenAIRE]

[15] Dang VD, Jella KK, Ragheb RRT, et al. Lipidomic and proteomic analysis of exosomes from mouse cortical collecting duct cells. FASEB J. 2017;31(12):5399-5408.

Available from: http://www.fasebj.org/doi/10.1096/fj.

201700417R [16] Klingeborn M, Dismuke WM, Skiba NP, et al.

327 references, page 1 of 22
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