High reproducibility of two-dimensional liquid chromatography using pH-driven fractionation with a pressure-resistant electrode.: High reproducibility of two-dimensional liquid chromatography using pH-driven fractionation with a pressure-resistant electrode.

Article English OPEN
Suberbiellle , Elsa; Gonzales-Dunia , Daniel; Pont , Frédéric;
(2008)
  • Publisher: Elsevier
  • Related identifiers: doi: 10.1016/j.jchromb.2008.06.043
  • Subject: Non-porous silica | MESH: Animals | Reverse phase high-performance liquid chromatography | MESH : Animals | Chromatogram comparison | [ SDV.BBM.GTP ] Life Sciences [q-bio]/Biochemistry, Molecular Biology/Genomics [q-bio.GN] | HPLC | [SDV.BBM.GTP]Life Sciences [q-bio]/Biochemistry, Molecular Biology/Genomics [q-bio.GN] | Two-dimensional liquid chromatography | Peak concordance | MESH: Cercopithecus aethiops | PF2D | MESH: Hydrogen-Ion Concentration | Proteomics | MESH : Vero Cells | Reproducibility | MESH : Cercopithecus aethiops | MESH: Proteomics | MESH : Chromatography, Liquid | MESH : Proteomics | MESH: Chromatography, Liquid | MESH: Reproducibility of Results | MESH : Hydrogen-Ion Concentration | MESH : Reproducibility of Results | Chromatofocusing | MESH: Vero Cells

International audience; Automated two-dimensional liquid chromatography using the PF2D system from Beckman Coulter provides a fractionation platform well suited for differential proteomic studies. To date, the reliability and reproducibility of PF2D has not been accurat... View more
  • References (7)

    3. Issaq, H.J. and Veenstra, T.D. (2007) The role of electrophoresis in disease biomarker discovery. Electrophoresis 28, 1980-1988 4. Skalnikova, H., Halada, P., Dzubak, P., Hajduch, M. and Kovarova, H. (2005) Protein fingerprints of anti-cancer effects of cyclin-dependent kinase inhibition: 320 identification of candidate biomarkers using 2-d liquid phase separation coupled to mass spectrometry. Technol. Cancer Res. Treat. 4, 447-454 5. Lubman, D.M., Kachman, M.T., Wang, H., Gong, S., Yan, F., Hamler, R.L., O'Neil, K.A., Zhu, K., Buchanan, N.S. and Barder, T.J. (2002) Two-dimensional liquid separations-mass mapping of proteins from human cancer cell lysates. J.

    325 Chromatogr. B Analyt. Technol. Biomed. Life Sci. 782, 183-196

    6. Zhu, K., Zhao, J., Lubman, D.M., Miller, F.R. and Barder, T.J. (2005) Protein pI shifts due to posttranslational modifications in the separation and characterization of proteins. Anal. Chem. 77, 2745-2755

    McDonald, T., Sheng, S., Stanley, B., Chen, D., Ko, Y., Cole, R.N., Pedersen, 8. Li, X., Gong, Y., Wang, Y., Wu, S., Cai, Y., He, P., Lu, Z., Ying, W., Zhang, Y., 335 Jiao, L., He, H., Zhang, Z., He, F., Zhao, X. and Qian, X. (2005) Comparison of alternative analytical techniques for the characterisation of the human serum proteome in hupo plasma proteome project. Proteomics 5, 3423-3441 9. Park, K.H., Lipuma, J.J. and Lubman, D.M. (2007) Comparative proteomic analysis of b. cenocepacia using two-dimensional liquid separations coupled with 340 mass spectrometry. Anal. Chim. Acta 592, 91-100

    10. Soldi, M., Sarto, C., Valsecchi, C., Magni, F., Proserpio, V., Ticozzi, D. and Mocarelli, P. (2005) Proteome profile of human urine with two-dimensional liquid phase fractionation. Proteomics 5, 2641-2647 11. Yan, F., Subramanian, B., Nakeff, A., Barder, T.J., Parus, S.J. and Lubman, 345 D.M. (2003) A comparison of drug-treated and untreated hct-116 human colon adenocarcinoma cells using a 2-d liquid separation mapping method based upon chromatofocusing pI fractionation. Anal. Chem. 75, 2299-2308 12. Old, W.M., Meyer-Arendt, K., Aveline-Wolf, L., Pierce, K.G., Mendoza, A., Sevinsky, J.R., Resing, K.A. and Ahn, N.G. (2005) Comparison of label-free methods 350 for quantifying human proteins by shotgun proteomics. Mol. Cell Proteomics 4, 1487-1502

    13. Dauly, C., Perlman, D.H., Costello, C.E. and McComb, M.E. (2006) Protein separation and characterization by np-rp-hplc followed by intact maldi-tof mass 21

    355 14. Cussac, D., Pichereaux, C., Colomba, A., Capilla, F., Pont, F., Gaits-Iacovoni, F., Lamant, L., Espinos, E., Burlet-Schiltz, O., Monsarrat, B., Delsol, G. and Payrastre, B. (2006) Proteomic analysis of anaplastic lymphoma cell lines: identification of potential tumour markers. Proteomics 6, 3210-3222

  • Similar Research Results (1)
  • Metrics
Share - Bookmark