High reproducibility of two-dimensional liquid chromatography using pH-driven fractionation with a pressure-resistant electrode. : High reproducibility of two-dimensional liquid chromatography using pH-driven fractionation with a pressure-resistant electrode.
Suberbiellle , Elsa
Gonzales-Dunia , Daniel
Pont , Frédéric
- Publisher: Elsevier
Non-porous silica | Proteomics | MESH : Vero Cells | Reverse phase high-performance liquid chromatography | Reproducibility | MESH : Cercopithecus aethiops | MESH : Animals | Chromatogram comparison | MESH : Chromatography, Liquid | [ SDV.BBM.GTP ] Life Sciences [q-bio]/Biochemistry, Molecular Biology/Genomics [q-bio.GN] | MESH : Proteomics | HPLC | MESH : Hydrogen-Ion Concentration | MESH : Reproducibility of Results | Two-dimensional liquid chromatography | Peak concordance | Chromatofocusing | PF2D
International audience; Automated two-dimensional liquid chromatography using the PF2D system from Beckman Coulter provides a fractionation platform well suited for differential proteomic studies. To date, the reliability and reproducibility of PF2D has not been accurat...