High reproducibility of two-dimensional liquid chromatography using pH-driven fractionation with a pressure-resistant electrode.: High reproducibility of two-dimensional liquid chromatography using pH-driven fractionation with a pressure-resistant electrode.

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Suberbiellle , Elsa; Gonzales-Dunia , Daniel; Pont , Frédéric;
  • Publisher: Elsevier
  • Related identifiers: doi: 10.1016/j.jchromb.2008.06.043
  • Subject: Non-porous silica | MESH: Animals | Reverse phase high-performance liquid chromatography | MESH : Animals | Chromatogram comparison | [ SDV.BBM.GTP ] Life Sciences [q-bio]/Biochemistry, Molecular Biology/Genomics [q-bio.GN] | HPLC | [SDV.BBM.GTP]Life Sciences [q-bio]/Biochemistry, Molecular Biology/Genomics [q-bio.GN] | Two-dimensional liquid chromatography | Peak concordance | MESH: Cercopithecus aethiops | PF2D | MESH: Hydrogen-Ion Concentration | Proteomics | MESH : Vero Cells | Reproducibility | MESH : Cercopithecus aethiops | MESH: Proteomics | MESH : Chromatography, Liquid | MESH : Proteomics | MESH: Chromatography, Liquid | MESH: Reproducibility of Results | MESH : Hydrogen-Ion Concentration | MESH : Reproducibility of Results | Chromatofocusing | MESH: Vero Cells

International audience; Automated two-dimensional liquid chromatography using the PF2D system from Beckman Coulter provides a fractionation platform well suited for differential proteomic studies. To date, the reliability and reproducibility of PF2D has not been accurat... View more
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