Analysis of Pseudomonas aeruginosa Cell Envelope Proteome by Capture of Surface-Exposed Proteins on Activated Magnetic Nanoparticles

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Davide Vecchietti; Dario Di Silvestre; Matteo Miriani; Francesco Bonomi; Mauro Marengo; Alessandra Bragonzi; Lara Cova; Eleonora Franceschi; Pierluigi Mauri; Giovanni Bertoni;
(2012)
  • Publisher: Public Library of Science
  • Journal: PLoS ONE,volume 7,issue 11,pagee51062 (issn: 1932-6203, eissn: 1932-6203)
  • Publisher copyright policies & self-archiving
  • Related identifiers: doi: 10.1371/journal.pone.0051062, pmc: PMC3511353
  • Subject: Settore BIO/10 - Biochimica | Transmembrane Proteins | Microbial Pathogens | Research Article | Biology | Bacterial Physiology | Proteomics | Microbiology | Medicine | Bacterial Pathogens | Gram Negative | Prokaryotic Models | Proteome | Q | R | Bacteriology | Model Organisms | Microbial Physiology | Proteomic Databases | Settore BIO/19 - Microbiologia Generale | Proteins | Science | Biochemistry | Spectrometric Identification of Proteins

We report on specific magneto-capturing followed by Multidimensional Protein Identification Technology (MudPIT) for the analysis of surface-exposed proteins of intact cells of the bacterial opportunistic pathogen Pseudomonas aeruginosa. The magneto-separation of cell en... View more
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