publication . Article . Other literature type . 2003

Mechanical Defects of Muscle Fibers with Myosin Light Chain Mutants that Cause Cardiomyopathy

Roopnarine, Osha;
Open Access English
  • Published: 01 Apr 2003 Journal: Biophysical Journal, volume 84, issue 4, pages 2,440-2,449 (issn: 0006-3495, Copyright policy)
  • Publisher: Biophysical Society
Abstract
Familial hypertrophic cardiomyopathy is a disease caused by single mutations in several sarcomeric proteins, including the human myosin ventricular regulatory light chain (vRLC). The effects of four of these mutations (A13T, F18L, E22K, and P95A) in vRLC on force generation were determined as a function of Ca2+ concentration. The endogenous RLC was removed from skinned rabbit psoas muscle fibers, and replaced with either rat wildtype vRLC or recombinant rat vRLC (G13T, F18L, E22K, and P95A). Compared to fibers with wildtype rat vRLC, the E22K mutant increased Ca sensitivity of force generation, whereas the G13T and F18L mutants decreased the Ca sensitivity, and ...
Subjects
free text keywords: Muscles and Contractility, Biophysics
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publication . Article . Other literature type . 2003

Mechanical Defects of Muscle Fibers with Myosin Light Chain Mutants that Cause Cardiomyopathy

Roopnarine, Osha;