publication . Article . 2012

Dilemmas in the reliable estimation of the in-vitro cell viability in magnetic nanoparticle engineering: which tests and what protocols?

Hoskins, Clare; Wang, Lijun; Cheng, Woei Ping; Cuschieri, Alfred;
Open Access English
  • Published: 01 Jan 2012 Journal: Nanoscale Research Letters, volume 7, issue 1, page 77 (issn: 1556-276X, Copyright policy)
  • Publisher: Springer Nature
Magnetic nanoparticles [MNPs] made from iron oxides have many applications in biomedicine. Full understanding of the interactions between MNPs and mammalian cells is a critical issue for their applications. In this study, MNPs were coated with poly(ethylenimine) [MNP-PEI] and poly(ethylene glycol) [MNP-PEI-PEG] to provide a subtle difference in their surface charge and their cytotoxicity which were analysed by three standard cell viability assays: 3-(4,5-dimethylthiazol-2-yl)-5-(3-carboxymethoxyphenyl)-2-(4-sulfophenyl)-2H-tetrazolium [MTS], CellTiter-Blue and CellTiter-Glo (Promega, Southampton, UK) in SH-SY5Y and RAW 264.7 cells The data were validated by trad...
free text keywords: Materials Science(all), Condensed Matter Physics, Nano Express, magnetic nanoparticle, cellular interaction, cell viability assay, zeta potential., General Materials Science, Viability assay, Cytotoxicity, Lipid peroxidation, chemistry.chemical_compound, chemistry, Trypan blue, Nanotechnology, Cell membrane, medicine.anatomical_structure, medicine, Biocompatibility, Magnetic nanoparticles, PEGylation
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