Identification of Cysteine Proteases and Screening of Cysteine Protease Inhibitors in Biological Samples by a Two-Dimensional Gel System of Zymography and Reverse Zymography

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Eiichi Saitoh; Shinya Yamamoto; Eishiro Okamoto; Yoshimi Hayakawa; Takashi Hoshino; Ritsuko Sato; Satoko Isemura; Sadami Ohtsubo; Masayuki Taniguchi;
  • Publisher: Libertas Academica
  • Journal: Analytical Chemistry Insights,volume 2,pages51-59 (issn: 1177-3901, eissn: 1177-3901)
  • Publisher copyright policies & self-archiving
  • Related identifiers: pmc: PMC2716818
  • Subject: Broad bean lectin | Reverse zymography | Cysteine protease inhibitors | QD71-142 | Original Research | Analytical chemistry | Cysteine proteases | Two-dimensional gel electrophoresis | Zymography

We have developed a two-dimensional (2D-) gel system of zymography and reverse zymography for the detection and characterization of proteases and protease inhibitors. Isoelectric focusing (IEF) agarose gels with pH gradients were employed for separation in the fi rst-di... View more
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