publication . Article . 2010

Effect of bone morphogenetic protein-7 (BMP-7) on in vitro survival of caprine preantral follicles

G. M. Silva; Valdevane Rocha Araújo; José Ricardo de Figueiredo; D.M. Magalhães; Cleidson Manoel Gomes da Silva; Ana Paula Ribeiro Rodrigues; José Roberto Viana Silva; Sônia Nair Báo;
Open Access English
  • Published: 01 Apr 2010
  • Publisher: Colégio Brasileiro de Patologia Animal - CBPA. Empresa Brasileira de Pesquisa Agropecuária (EMBRAPA)
  • Country: Brazil
Abstract
This study was conducted in order to verify the effect of different concentrations of BMP-7 in the in vitro survival and development of caprine preantral follicles. Fragments of caprine ovarian cortical tissue were cultured for 1 or 7 days in Minimum Essential Medium (MEM+) supplemented with different concentrations of BMP-7 (1, 10, 50 or 100ng/ml). Non-cultured fragments or those cultured for 1 or 7 days were processed for classical histology and transmission electron microscopy (TEM). Parameters such as follicular survival, activation and growth were evaluated. The results showed that, after 1 or 7 days of culture, the percentage of morphologically normal foll...
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doi: 10.1590/s0100-736x2010000400004 , 10.1590/s0100-736x2010000400004.
Subjects
free text keywords: Ovary, preantral follicles, growth factor, bone morphogenetic protein-7, BMP-7, goats, Ovário, folículos pré-antrais, fator de crescimento, proteína morfogenética óssea 7, caprinos, General Veterinary, Ovários, Caprino, Veterinary medicine, SF600-1100, Endocrinology, medicine.medical_specialty, medicine, In vitro, Histology, Bone morphogenetic protein 7, Internal medicine, medicine.anatomical_structure, Oocyte, Ultrastructure, medicine.medical_treatment, Biology, Follicular phase
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