Behavior of the Meat-Borne Bacterium Lactobacillus sakei during Its Transit through the Gastrointestinal Tracts of Axenic and Conventional Mice ▿ †

Article, Other literature type English OPEN
Chiaramonte, Fabrizio ; Blugeon, Sébastien ; Chaillou, Stéphane ; Langella, Philippe ; Zagorec, Monique (2009)
  • Publisher: American Society for Microbiology (ASM)
  • Related identifiers: doi: 10.1128/AEM.02868-08
  • Subject: strain | excrément | [ SDV ] Life Sciences [q-bio] | diversity;expression;growth;strain;adaptation;identification;mouse gut;human feces;escherichia-coli;lactic-acid bacteria | escherichia coli | diversité | expression | croissance | escherichia-coli | adaptation | mouse gut | diversity | souris | identification | lactic-acid bacteria | souche | growth | intestin | human feces | Food Microbiology | bactérie lactique
    mesheuropmc: food and beverages

A Lactobacillus sakei strain named FLEC01 was isolated from human feces and characterized genotypically. Comparison of the genetic features of this strain with those of both the meat-borne L. sakei strain 23K and another human isolate, LTH5590, showed that they belong to different but closely related clusters. The three L. sakei strains did not persist and only transited through the gastrointestinal tracts (GITs) of conventional C3H/HeN mice. In contrast, they all colonized the GITs of axenic mice and rapidly reached a population of 10(9) CFU/g of feces, which remained stable until day 51. Five days after mice were fed, a first subpopulation, characterized by small colonies, appeared and reached 50% of the total L. sakei population in mice. Fifteen to 21 days after feeding, a second subpopulation, characterized by rough colonies, appeared. It coexisted with the two other populations until day 51, and its cell shapes were also affected, suggesting a dysfunction of the cell division or cell wall. No clear difference between the behaviors of the meat-borne strain and the two human isolates in both conventional and axenic mice was observed, suggesting that L. sakei is a food-borne bacterium rather than a commensal one and that its presence in human feces originates from diet. Previous observations of Escherichia coli strains suggest that the mouse GIT environment could induce mutations to increase their survival and colonization capacities. Here, we observed similar mutations concerning a food-grade gram-positive bacterium for the first time.
  • References (39)
    39 references, page 1 of 4

    1. Alpert, C. A., A. M. Crutz-Le Coq, C. Malleret, and M. Zagorec. 2003. Characterization of a theta-type plasmid from Lactobacillus sakei: a potential basis for low-copy-number vectors in lactobacilli. Appl. Environ. Microbiol. 69:5574-5584.

    2. Ammor, S., C. Rachman, S. Chaillou, H. Pr´evost, X. Dousset, M. Zagorec, E. Dufour, and I. Chevallier. 2005. Phenotypic and genotypic identification of lactic acid bacteria isolated from a small-scale facility producing traditional dry sausage. Food Microbiol. 22:373-382.

    3. Ammor, S., E. Dufour, M. Zagorec, S. Chaillou, and I. Chevallier. 2005. Characterization and selection of Lactobacillus sakei strains isolated from traditional dry-sausage. Food Microbiol. 22:529-538.

    4. Anderson, D. G., and L. L. McKay. 1983. Simple and rapid method for isolating large plasmid DNA from lactic streptococci. Appl. Environ. Microbiol. 46:549-555.

    5. Balca´zar, J. L., I. de Blas, I. Ruiz-Zarzuela, D. Vendrell, A. C. Calvo, I. Marquez, O. Girones, and J. L. Muzquiz. 2007. Changes in intestinal microbiota and humoral immune response following probiotic administration in brown trout (Salmo trutta). Br. J. Nutr. 97:522-527.

    6. Barton, B. M., G. P. Harding, and A. J. Zuccarelli. 1995. A general method for detecting and sizing large plasmids. Anal. Biochem. 226:235-240.

    7. Berthier, F., M. Zagorec, M. Champomier-Verg`es, S. D. Ehrlich, and F. Morel-Deville. 1996. High-frequency transformation of Lactobacillus sake by electroporation. Microbiology 142:1273-1279.

    8. Bredholt, S., T. Nesbakken, and A. Holck. 2001. Industrial application of an antilisterial strain of L. sakei as a protective culture and its effect on the sensory acceptability of cooked, sliced, vacuum-packaged meat products. Int. J. Food Microbiol. 66:191-196.

    9. Broda, D. M., P. A. Lawson, R. G. Bell, and D. R. Musgrave. 1999. Clostridium frigidicans sp. nov., a psychrotolerant bacterium associated with 'blown pack' spoilage of vacuum-packed meats. Int. J. Syst. Bacteriol. 49:1539-1550.

    10. Chaillou, S., M. C. Champomier-Verg`es, M. Cornet, A. M. Crutz-Le Coq, A. M. Dudez, V. Martin, S. Beaufils, R. Bossy, E. Darbon-Rong`ere, V. Loux, and M. Zagorec. 2005. Complete genome sequence of the meat-borne lactic acid bacterium Lactobacillus sakei 23K. Nat. Biotechnol. 23:1527-1533.

  • Related Research Results (1)
  • Metrics
    No metrics available
Share - Bookmark