Resistin in dairy cows: Plasma concentrations during early lactation, expression and potential role in adipose tissue

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Reverchon, Maxime ; Ramé, Christelle ; Cognié, Juliette ; Briant, Eric ; Elis, Sébastien ; Guillaume, Daniel ; Dupont, Joëlle (2014)
  • Publisher: Public Library of Science (PLoS)
  • Journal: PLoS ONE, volume 9, issue 3 (issn: 1932-6203, eissn: 1932-6203)
  • Related identifiers: pmc: PMC3968062, doi: 10.1371/journal.pone.0093198
  • Subject: Animal Performance | Endocrine System | Animal Breeding | Signaling cascades | Research Article | Molecular Cell Biology | Anatomy | Hormones | adipokine | AKT signaling cascade | Agriculture | insuline | arn messager | Endocrine Physiology | vache laitière | phosphorylation | tissu adipeux | extraction de l'arn | Biology and life sciences | Signal transduction | Physiology | Medicine | Q | R | Cell biology | lactation | Biochemistry | Cell signaling | Science | bovin | adipocyte | Medicine and Health Sciences | Animal Management | Diabetic Endocrinology | Insulin signaling cascade | Endocrinology
    mesheuropmc: nutritional and metabolic diseases | hormones, hormone substitutes, and hormone antagonists

Resistin is an adipokine that has been implicated in energy metabolism regulation in rodents but has been little studied in dairy cows. We determined plasma resistin concentrations in early lactation in dairy cows and investigated the levels of resistin mRNA and protein in adipose tissue and the phosphorylation of several components of insulin signaling pathways one week post partum (1 WPP) and at five months of gestation (5 MG). We detected resistin in mature bovine adipocytes and investigated the effect of recombinant bovine resistin on lipolysis in bovine adipose tissue explants. ELISA showed that plasma resistin concentration was low before calving, subsequently increasing and reaching a peak at 1 WPP, decreasing steadily thereafter to reach pre-calving levels at 6 WPP. Plasma resistin concentration was significantly positively correlated with plasma non esterified fatty acid (NEFA) levels and negatively with milk yield, dry matter intake and energy balance between WPP1 to WPP22. We showed, by quantitative RT-PCR and western blotting, that resistin mRNA and protein levels in adipose tissue were higher at WPP1 than at 5 MG. The level of phosphorylation of several early and downstream insulin signaling components (IRβ, IRS-1, IRS-2, Akt, MAPK ERK1/2, P70S6K and S6) in adipose tissue was also lower at 1 WPP than at 5 MG. Finally, we showed that recombinant bovine resistin increased the release of glycerol and mRNA levels for ATGL (adipose triglyceride lipase) and HSL (hormone-sensitive lipase) in adipose tissue explants. Overall, resistin levels were high in the plasma and adipose tissue and were positively correlated with NEFA levels after calving. Resistin is expressed in bovine mature adipocytes and promotes lipid mobilization in adipose explants in vitro.
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