Urine storage under refrigeration preserves the sample in chemical, cellularity and bacteriuria analysis of ACS

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Karen Cristina Barcellos Ribeiro ; Bruno Rotondo Levenhagem Serabion ; Eduardo Lima Nolasco ; Chislene Pereira Vanelli ; Harleson Lopes de Mesquita ; José Otávio do Amaral Corrêa (2013)
  • Publisher: Sociedade Brasileira de Patologia Clínica
  • Journal: Jornal Brasileiro de Patologia e Medicina Laboratorial (issn: 1678-4774)
  • Related identifiers: doi: 10.1590/S1676-24442013000600006
  • Subject: pyuria | hematuria Introduction | piúria | RB1-214 | urine storage | urinalysis | uroanálise | Pathology | armazenamento da urina | hematúria | cristalúria | crystalluria

INTRODUCTION: The analysis of urine abnormal constituents and sediment (ACS) comprises tests of great diagnostic and prognostic value in clinical practice. When the analysis of ACS cannot be performed within two hours after collection, the sample must be preserved in order to avoid pre-analytical interferences. Refrigeration is the most applied technique due to its cost effectiveness. Moreover, it presents fewer inconveniences when compared to chemical preservation. However, changes in ACS may also occur in samples under refrigeration. OBJECTIVE: To analyze the influence of refrigeration at 2 to 8ºC on the storage of urine samples within 24 hours. MATERIAL AND METHOD: A total of 80 urine samples were selected from patients admitted at Universidade Federal de Juiz de Fora (UFJF) university hospital, which were tested for ACS at room temperature and stored under refrigeration for 6, 12 and 24 hours. RESULTS: The results showed that refrigeration proved to be effective when compared to samples kept at room temperature, inasmuch as the physical, chemical, microbial and cellularity features were preserved. Nevertheless, crystalluria was present after a 6- hour storage period. CONCLUSION: The tests revealed that cooling preserved cellularity and chemical characteristics of urine samples for up to 12 hours. Nonetheless, the precipitation of crystals was evident in this storage method. Thus, the possible consequences of storing urine samples for ACS test under these conditions should be included in the analysis report.