Transcriptome changes in apple peel tissues during CO2 injury symptom development under controlled atmosphere storage regimens

Article English OPEN
Johnson, Franklin T ; Zhu, Yanmin (2015)
  • Publisher: Nature Publishing Group
  • Journal: Horticulture Research, volume 2, page 15,061 (issn: 2052-7276, eissn: 2052-7276)
  • Related identifiers: pmc: PMC4817516, doi: 10.1038/hortres.2015.61
  • Subject: Article
    mesheuropmc: food and beverages | fungi

Apple (Malus × domestica Borkh.) is one of the most widely cultivated tree crops, and fruit storability is vital to the profitability of the apple fruit industry. Fruit of many apple cultivars can be stored for an extended period due to the introduction of advanced storage technologies, such as controlled atmosphere (CA) and 1-methylcyclopropane (1-MCP). However, CA storage can cause external CO2 injury for some apple cultivars. The molecular changes associated with the development of CO2 injury are not well elucidated. In this study, the global transcriptional regulations were investigated under different storage conditions and during development of CO2 injury symptoms on ‘Golden Delicious’ fruit. Fruit peel tissues under three different storage regimens, regular cold atmosphere, CA and CA storage and 1-MCP application were sampled at four storage durations over a 12-week period. Fruit physiological changes were affected differently under these storage regimens, and CO2 injury symptoms were detectable 2 weeks after CA storage. Identification of the differentially expressed genes and a gene ontology enrichment analysis revealed the specific transcriptome changes associated with each storage regimen. Overall, a profound transcriptome change was associated with CA storage regimen as indicated by the large number of differentially expressed genes. The lighter symptom was accompanied by reduced transcriptome changes under the CA storage and 1-MCP application regimen. Furthermore, the higher enrichment levels in the functional categories of oxidative stress response, glycolysis and protein post-translational modification were only associated with CA storage regime; therefore, these processes potentially contribute to the development of external CO2 injury or its symptom in apple.
  • References (49)
    49 references, page 1 of 5

    Hummer KE, Janick J. Rosaceae: taxonomy, economic importance, genomics. In: Folta K, Gardiner S, editors. Genetics and Genomics of Rosaceae. New York: Springer; 2009. pp 1–17.

    Mattheis JP. How 1-methylcyclopropene has altered the Washington State apple industry. Hort Sci 2008; 43: 99–101.

    Sisler EC. The discovery and development of compounds counteracting ethylene at the receptor level. Biotech Adv 2006; 24: 357–367.

    Watkins CB, Nock JF. SmartFreshTM (1-MCP)—the good and bad as we head into the 2004 season. New York Fruit Quart 2004; 12: 3–8.

    Kader AA, Rolle RS. The role of post-harvest management in assuring the quality and safety of horticultural produce. Food Agri Org 2004; 152: 35–42.

    Kidd F, West C, Kidd MN. Gas storage of fruit. Special Rep No 30, Sci Industrial Research Dept, London, Report No. 87.

    Rothan C, Duret S, Chevalier C, Raymond P. Suppression of ripening-associated gene expression in tomato fruits subjected to a high CO 2 concentration. Plant Physiol 199; 114: 255–263.

    Smock RM. Controlled atmosphere storage of fruits. Hort Rev 1979; 1: 301–336.

    Burmeister DM, Dilley DR. A scald-like controlled atmosphere storage disorder of Empire apples—a chilling injury induced by CO 2. Postharvest Bio Technol 1995; 6: 1–7.

    Elgar HJ, Burmeister DM, Watkins CB. Storage and handling effects on a CO 2-related internal browning disorder of ‘Braeburn’ apples. Hort Sci 1998; 33: 719–722.

  • Metrics
    No metrics available
Share - Bookmark