Genetic variability and discrimination of low doses of Toxocara spp. from public areas soil inferred by loop-mediated isothermal amplification assay as a field-friendly molecular tool

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Maryam Ozlati ; Adel Spotin ; Abbas Shahbazi ; Mahmoud Mahami-Oskouei ; Teimour Hazratian ; Mohammad Adibpor ; Ehsan Ahmadpour ; Afsaneh Dolatkhah ; Paria Khoshakhlagh (2016)
  • Publisher: Veterinary World
  • Journal: Veterinary World, volume 9, issue 12, pages 1,471-1,477 (issn: 0972-8988, eissn: 2231-0916)
  • Related identifiers: pmc: PMC5234066, doi: 10.14202/vetworld.2016.1471-1477
  • Subject: gene flow | Research Article | Toxocara spp. | Iran | Animal culture | soil | SF600-1100 | Toxocara spp | Veterinary medicine | loop-mediated isothermal amplification | polymerase chain reaction | SF1-1100
    mesheuropmc: parasitic diseases

Abstract: Aim: One of the main diagnostic problems of conventional polymerase chain reaction (PCR) is indiscrimination of low parasitic loads in soil samples. The aim of this study is to determine the genetic diversity and identification of Toxocara spp. from public are... View more
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